FastQCFastQC Report
Thu 26 May 2016
SRR1041738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9784905
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT528480.5400972211789485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT425890.43525205405673334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT349400.3570806257189007No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC98230.10038932416819582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93800.030.0181241
GTATCAA214800.020.5153641
TCGAAAT23000.017.13260826
GTCGAAA23150.017.10151325
GTGCGAA23400.016.99786411
TGCGAAC23800.016.63445312
CCGTAGT23900.016.56485420
CCCGTAG24750.016.2949519
TCGTCTA15700.016.14331227
TAGTCGA25300.016.08695623
CCCCGTA24950.015.86773418
GTAGTCG25700.015.47665422
AGTCGAA26400.015.34659124
CGTCTAT16500.015.24848628
CGTAGTC26600.015.02255721
GTTCGTC16450.014.6200625
TACGCAT13950.014.58781419
ACCCCGT28850.013.78682817
TGTTCGT17850.013.78431424
TATCAAC315700.013.7592652
Produced by FastQC (version 0.11.5)