##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041738_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9784905
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.781105590703234	34.0	31.0	34.0	31.0	34.0
2	33.00421046499685	34.0	31.0	34.0	31.0	34.0
3	33.061934684087376	34.0	33.0	34.0	31.0	34.0
4	36.449648514727535	37.0	37.0	37.0	35.0	37.0
5	36.409674697914795	37.0	37.0	37.0	35.0	37.0
6	36.47526797654142	37.0	37.0	37.0	35.0	37.0
7	36.435904896368434	37.0	37.0	37.0	35.0	37.0
8	36.42123924555221	37.0	37.0	37.0	35.0	37.0
9	38.16381855521336	39.0	39.0	39.0	37.0	39.0
10	38.14044827210893	39.0	39.0	39.0	37.0	39.0
11	38.20755285820353	39.0	39.0	39.0	37.0	39.0
12	38.10737436898979	39.0	38.0	39.0	37.0	39.0
13	38.12677823647751	39.0	38.0	39.0	37.0	39.0
14	39.036255334109015	40.0	39.0	40.0	37.0	40.0
15	39.05778042811862	40.0	39.0	40.0	37.0	40.0
16	39.04379838128219	40.0	39.0	40.0	37.0	40.0
17	39.03593811079413	40.0	39.0	40.0	37.0	40.0
18	38.99526791522248	40.0	39.0	40.0	37.0	40.0
19	39.00200420954521	40.0	39.0	40.0	37.0	40.0
20	38.95168016449828	40.0	39.0	40.0	37.0	40.0
21	38.90399538881573	40.0	39.0	40.0	37.0	40.0
22	38.8488641432901	40.0	39.0	40.0	36.0	40.0
23	38.78812773348336	40.0	39.0	40.0	36.0	40.0
24	38.73676187965034	40.0	39.0	40.0	36.0	40.0
25	38.702792720011075	40.0	39.0	40.0	36.0	40.0
26	38.58379902513106	40.0	39.0	40.0	36.0	40.0
27	38.51730221192745	40.0	38.0	40.0	35.0	40.0
28	38.445609538365474	40.0	38.0	40.0	35.0	40.0
29	38.378203058690914	40.0	38.0	40.0	35.0	40.0
30	38.291414173157534	40.0	38.0	40.0	35.0	40.0
31	38.19749093118431	40.0	38.0	40.0	35.0	40.0
32	38.27890664242525	40.0	38.0	40.0	35.0	40.0
33	38.37283427892248	40.0	38.0	40.0	35.0	40.0
34	38.376242794385846	40.0	38.0	40.0	35.0	40.0
35	38.40845271364413	40.0	38.0	40.0	35.0	40.0
36	38.39051815015067	40.0	38.0	40.0	35.0	40.0
37	38.34970078912365	40.0	38.0	40.0	35.0	40.0
38	38.3104543171344	40.0	38.0	40.0	35.0	40.0
39	38.25590774769913	40.0	38.0	40.0	35.0	40.0
40	38.21955225932189	40.0	38.0	40.0	35.0	40.0
41	38.165797623993285	40.0	38.0	40.0	35.0	40.0
42	38.080978200605934	40.0	38.0	40.0	35.0	40.0
43	37.75242386103902	40.0	38.0	40.0	34.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	5.0
13	3.0
14	4.0
15	7.0
16	16.0
17	39.0
18	60.0
19	158.0
20	333.0
21	764.0
22	1575.0
23	3177.0
24	5791.0
25	9583.0
26	15113.0
27	23221.0
28	33907.0
29	48613.0
30	66392.0
31	88063.0
32	115501.0
33	152854.0
34	215750.0
35	315976.0
36	524590.0
37	1144122.0
38	5229127.0
39	1790158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.815499486198384	18.419013776832784	13.320885588567288	24.444601148401542
2	17.34590167201419	19.948819124968512	37.772599733978005	24.9326794690393
3	18.965978719261965	23.130382972548023	29.936192533294907	27.967445774895104
4	14.093504229218373	16.16369295358514	35.39740038354997	34.34540243364652
5	14.879674355550717	36.20069893371473	33.87832584986773	15.041300860866816
6	33.94383491715045	35.57938477685782	15.67596210693921	14.80081819905252
7	29.496852549922558	31.209858450337535	20.255628439928643	19.037660559811258
8	27.224955173300096	34.826909407909426	18.36514508827628	19.582990330514193
9	26.962857585229493	15.157561570602882	18.858742113490116	39.02083873067751
10	16.49216829391803	27.91074619528754	31.328714995189017	24.268370515605415
11	35.79600415129222	22.395710535769126	21.974071286333388	19.834214026605267
12	23.67903418581989	24.34005235615471	28.878921154574318	23.10199230345108
13	29.713155109834993	20.95586007222349	25.03172999635663	24.299254821584878
14	22.966630743987807	20.136199584972978	26.31038318716431	30.586786483874906
15	24.813209734790476	27.769201642734398	23.15626978493915	24.26131883753598
16	24.382944954498793	27.179129485672064	24.157219717513865	24.28070584231528
17	23.815795861073767	26.616589532550393	25.785891636147717	23.781722970228124
18	24.49445344640546	25.81860529049592	26.371395532199855	23.31554573089877
19	25.7721153143541	25.339847448697768	26.390496381927058	22.497540855021075
20	24.90491220916299	25.44489701228576	26.263188043215546	23.387002735335706
21	24.775559905793667	25.217465064811563	26.620227789641287	23.38674723975348
22	24.422863584265762	24.983921663010523	26.513900748142166	24.079314004581548
23	23.944780250804683	24.92843824237435	27.08310402604829	24.04367748077268
24	23.757001217691943	25.26460910964389	26.956572393906736	24.021817278757432
25	24.155533446671175	25.251967188235348	26.886433746674086	23.70606561841939
26	23.926088194009036	25.489332803946485	26.94712927718767	23.637449724856808
27	24.188339079428978	25.71233956793653	26.422361790942272	23.676959561692218
28	24.256627938646314	25.381605646656762	26.523047489985853	23.83871892471107
29	24.27235624668814	25.419122617950812	26.447686513052503	23.860834622308545
30	24.120602090669248	25.74625916143284	26.492868351813332	23.64027039608458
31	24.248819993653488	25.20892129254193	26.736447620084203	23.80581109372038
32	24.289362032641094	25.373644404314604	26.527033221068574	23.809960341975728
33	24.432490657804035	25.355453118860122	26.53223511112269	23.679821112213148
34	24.4552706439153	25.14778630962692	26.306550753430923	24.09039229302686
35	24.291252699949563	25.774506753003735	26.217178398768308	23.717062148278394
36	24.361503765238396	25.365918217908096	26.629681126183648	23.642896890669864
37	24.657418748572418	25.097085766290018	26.33277481999059	23.912720665146978
38	24.257680580445083	25.436639395068223	26.420184968581705	23.885495055904986
39	24.149268694994994	25.346694730301415	26.68083134174527	23.823205232958315
40	24.406665164352642	25.285202053571293	26.676201761795337	23.631931020280728
41	24.61183833670332	25.248655965489704	26.48818767274695	23.65131802506003
42	24.608956346535813	25.215891212025056	26.319621907417602	23.855530534021536
43	24.47346192936978	24.861610817887346	26.414461867539845	24.250465385203025
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2617.0
1	2350.5
2	2084.0
3	4062.5
4	6041.0
5	6041.0
6	6809.0
7	7577.0
8	6146.5
9	4716.0
10	6467.0
11	8218.0
12	8218.0
13	14464.0
14	20710.0
15	43238.0
16	65766.0
17	70988.5
18	76211.0
19	76211.0
20	73016.0
21	69821.0
22	64642.5
23	59464.0
24	71850.5
25	84237.0
26	84237.0
27	100813.5
28	117390.0
29	140423.0
30	163456.0
31	193529.0
32	223602.0
33	223602.0
34	260404.5
35	297207.0
36	338677.0
37	380147.0
38	428920.0
39	477693.0
40	477693.0
41	529320.5
42	580948.0
43	636454.5
44	691961.0
45	744966.0
46	797971.0
47	797971.0
48	842961.5
49	887952.0
50	901194.5
51	914437.0
52	898209.5
53	881982.0
54	881982.0
55	841200.0
56	800418.0
57	724771.0
58	649124.0
59	571622.0
60	494120.0
61	494120.0
62	428270.0
63	362420.0
64	309475.0
65	256530.0
66	207830.5
67	159131.0
68	159131.0
69	127248.0
70	95365.0
71	78683.5
72	62002.0
73	49493.0
74	36984.0
75	36984.0
76	28864.0
77	20744.0
78	16377.0
79	12010.0
80	9504.5
81	6999.0
82	6999.0
83	5417.0
84	3835.0
85	2859.5
86	1884.0
87	1317.5
88	751.0
89	751.0
90	499.5
91	248.0
92	167.5
93	87.0
94	58.0
95	29.0
96	29.0
97	18.5
98	8.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9784905.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.76088714080879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.03254954991765	29.246213979372097
2	14.534194295643344	12.13921695325897
3	5.526036383507411	6.923165452429683
4	2.7574950172596573	4.606217528284925
5	1.688211313453727	3.525060106548883
6	1.1055891370101532	2.7702229904871114
7	0.7378757075957727	2.157004090419604
8	0.5672255945347627	1.8950275229395515
9	0.4279126874977088	1.6083012103840795
>10	2.3899078317667533	18.033100702528035
>50	0.15158335994250985	4.336816779557466
>100	0.06994484945672139	5.379750679552391
>500	0.00620479462728401	1.7775531079874132
>1k	0.005024385944670937	3.7883141108677125
>5k	1.7156428930311418E-4	0.4795932379715621
>10k+	7.35275525584775E-5	1.3344415474105973
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	52848	0.5400972211789485	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	42589	0.43525205405673334	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34940	0.3570806257189007	No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC	9823	0.10038932416819582	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.109911644517755E-5
2	0.0	0.0	0.0	2.0439646578071017E-5	5.109911644517755E-5
3	0.0	0.0	0.0	3.0659469867106526E-5	5.109911644517755E-5
4	0.0	0.0	0.0	8.175858631228407E-5	7.153876302324857E-5
5	1.0219823289035509E-5	0.0	0.0	1.124180561793906E-4	7.153876302324857E-5
6	2.0439646578071017E-5	0.0	0.0	1.124180561793906E-4	8.175858631228407E-5
7	2.0439646578071017E-5	0.0	0.0	1.124180561793906E-4	8.175858631228407E-5
8	2.0439646578071017E-5	0.0	0.0	1.124180561793906E-4	8.175858631228407E-5
9	2.0439646578071017E-5	0.0	0.0	1.5329734933553263E-4	8.175858631228407E-5
10	3.0659469867106526E-5	0.0	0.0	2.248361123587812E-4	8.175858631228407E-5
11	5.109911644517755E-5	0.0	0.0	2.5549558222588773E-4	8.175858631228407E-5
12	5.109911644517755E-5	0.0	0.0	2.7593522880395874E-4	9.197840960131958E-5
13	5.109911644517755E-5	1.0219823289035509E-5	0.0	2.963748753820298E-4	9.197840960131958E-5
14	6.131893973421305E-5	1.0219823289035509E-5	0.0	2.963748753820298E-4	1.6351717262456814E-4
15	6.131893973421305E-5	1.0219823289035509E-5	0.0	3.372541685381718E-4	1.6351717262456814E-4
16	9.197840960131958E-5	1.0219823289035509E-5	0.0	3.6791363840527833E-4	1.8395681920263917E-4
17	9.197840960131958E-5	1.0219823289035509E-5	0.0	3.7813346169431386E-4	2.043964657807102E-4
18	9.197840960131958E-5	1.0219823289035509E-5	0.0	3.9857310827238487E-4	2.043964657807102E-4
19	9.197840960131958E-5	1.0219823289035509E-5	0.0	4.087929315614204E-4	2.043964657807102E-4
20	1.226378794684261E-4	1.0219823289035509E-5	0.0	4.496722247175624E-4	2.043964657807102E-4
21	1.4307752604649713E-4	1.0219823289035509E-5	0.0	5.212109877408109E-4	2.043964657807102E-4
22	1.4307752604649713E-4	1.0219823289035509E-5	0.0	5.620902808969531E-4	2.043964657807102E-4
23	1.4307752604649713E-4	1.0219823289035509E-5	0.0	5.927497507640596E-4	2.7593522880395874E-4
24	1.5329734933553263E-4	1.0219823289035509E-5	0.0	6.949479836544147E-4	2.963748753820298E-4
25	1.6351717262456814E-4	1.0219823289035509E-5	0.0	7.664867466776632E-4	2.963748753820298E-4
26	1.6351717262456814E-4	1.0219823289035509E-5	0.0	9.095642727241603E-4	2.963748753820298E-4
27	1.6351717262456814E-4	1.0219823289035509E-5	0.0	0.0012774779111294386	2.963748753820298E-4
28	1.6351717262456814E-4	2.0439646578071017E-5	0.0	0.003127265926444866	2.963748753820298E-4
29	1.6351717262456814E-4	2.0439646578071017E-5	0.0	0.006683764431029223	2.963748753820298E-4
30	1.6351717262456814E-4	2.0439646578071017E-5	0.0	0.011384883143985558	2.963748753820298E-4
31	1.6351717262456814E-4	2.0439646578071017E-5	0.0	0.019039530787473155	3.270343452491363E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9380	0.0	30.018124	1
GTATCAA	21480	0.0	20.515364	1
TCGAAAT	2300	0.0	17.132608	26
GTCGAAA	2315	0.0	17.101513	25
GTGCGAA	2340	0.0	16.997864	11
TGCGAAC	2380	0.0	16.634453	12
CCGTAGT	2390	0.0	16.564854	20
CCCGTAG	2475	0.0	16.29495	19
TCGTCTA	1570	0.0	16.143312	27
TAGTCGA	2530	0.0	16.086956	23
CCCCGTA	2495	0.0	15.867734	18
GTAGTCG	2570	0.0	15.476654	22
AGTCGAA	2640	0.0	15.346591	24
CGTCTAT	1650	0.0	15.248486	28
CGTAGTC	2660	0.0	15.022557	21
GTTCGTC	1645	0.0	14.62006	25
TACGCAT	1395	0.0	14.587814	19
ACCCCGT	2885	0.0	13.786828	17
TGTTCGT	1785	0.0	13.784314	24
TATCAAC	31570	0.0	13.759265	2
>>END_MODULE