FastQCFastQC Report
Thu 26 May 2016
SRR1041737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5301715
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT256670.48412636288446287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT225880.42605081563230013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT204870.38642212944301985No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC98440.18567576718099707No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC81920.1545160386780504No Hit
ATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGGAGGAC63750.12024410968903458No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62260.11743369834100852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68950.030.8020321
TACGCAT13450.021.31970419
CTACGCA14500.019.77586218
ACGCATT15050.019.42192820
CGCTACG14700.019.38095316
CGGGTTA15500.018.14193534
GCTACGC16200.017.81481617
CGCATTC16450.017.43161221
GCTTTCG16200.017.24382811
GTATCAA124900.016.9447542
CGACGCT7900.016.62658129
CGCTTTC17400.016.26724210
TGAATCG13600.016.05147218
GAATCGC13550.015.9741719
CGCCCTA13350.015.93632923
AATCGCC13350.015.93632920
CTCGGGT18350.015.92915532
TCGAAAT24200.015.8243826
TCGGGTT18100.015.74033233
GTCGAAA24850.015.48490925
Produced by FastQC (version 0.11.5)