##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041737_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5301715
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.070878574197216	31.0	31.0	33.0	30.0	34.0
2	31.276156677603378	31.0	31.0	34.0	30.0	34.0
3	31.234530335938466	31.0	31.0	34.0	28.0	34.0
4	34.955772235965156	35.0	35.0	37.0	32.0	37.0
5	34.660461379006605	35.0	35.0	37.0	32.0	37.0
6	35.03682261306011	35.0	35.0	37.0	32.0	37.0
7	35.04008136989634	36.0	35.0	37.0	32.0	37.0
8	35.03369570035356	36.0	35.0	37.0	32.0	37.0
9	36.553971875138515	38.0	35.0	39.0	32.0	39.0
10	36.196888365368565	38.0	35.0	39.0	32.0	39.0
11	36.51716001331645	38.0	35.0	39.0	32.0	39.0
12	36.18755006634645	37.0	35.0	39.0	32.0	39.0
13	36.31704288140724	38.0	35.0	39.0	32.0	39.0
14	36.99136694446985	38.0	36.0	40.0	32.0	40.0
15	37.02626866966632	38.0	36.0	40.0	32.0	40.0
16	36.93741704335295	38.0	36.0	40.0	32.0	40.0
17	36.88988657443865	38.0	36.0	40.0	32.0	40.0
18	36.792972839920665	38.0	36.0	40.0	31.0	40.0
19	36.80650393316125	39.0	36.0	40.0	31.0	40.0
20	36.68955988769672	38.0	36.0	40.0	31.0	40.0
21	36.58954036571185	38.0	36.0	40.0	31.0	40.0
22	36.46097366606843	38.0	35.0	40.0	31.0	40.0
23	36.32903994273551	38.0	35.0	40.0	30.0	40.0
24	36.20070731829229	38.0	35.0	40.0	30.0	40.0
25	36.13913988209475	38.0	35.0	40.0	30.0	40.0
26	35.85472870571127	38.0	34.0	40.0	29.0	40.0
27	35.73130581330758	38.0	34.0	40.0	29.0	40.0
28	35.58076452619577	38.0	34.0	40.0	28.0	40.0
29	35.43860241450172	38.0	34.0	40.0	27.0	40.0
30	35.28194178676145	38.0	34.0	40.0	27.0	40.0
31	35.11461121542746	38.0	34.0	40.0	27.0	40.0
32	35.201613817415684	38.0	34.0	40.0	27.0	40.0
33	35.37121554063167	38.0	34.0	40.0	28.0	40.0
34	35.37545228289336	38.0	34.0	40.0	28.0	40.0
35	35.395977716644516	38.0	34.0	40.0	28.0	40.0
36	35.330108276284186	38.0	34.0	40.0	27.0	40.0
37	35.25914350356441	38.0	34.0	40.0	27.0	40.0
38	35.1619532547487	38.0	34.0	40.0	27.0	40.0
39	35.01792080487163	38.0	33.0	40.0	27.0	40.0
40	34.95193423260209	38.0	33.0	40.0	26.0	40.0
41	34.847749266039386	38.0	33.0	40.0	26.0	40.0
42	34.685090956416936	37.0	33.0	40.0	26.0	40.0
43	34.09762369346523	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	4.0
13	19.0
14	18.0
15	58.0
16	119.0
17	247.0
18	508.0
19	1051.0
20	1946.0
21	3820.0
22	6604.0
23	11111.0
24	17786.0
25	27440.0
26	40980.0
27	58663.0
28	81790.0
29	111632.0
30	149908.0
31	198200.0
32	263868.0
33	350592.0
34	471238.0
35	633180.0
36	831484.0
37	974615.0
38	971431.0
39	93402.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.07238884021491	24.82247725500145	14.036269395846437	20.068864508937203
2	23.235198421642806	24.0066469057654	32.54043267131485	20.217722001276943
3	24.912221799927003	24.336238368150685	28.64605132490147	22.10548850702084
4	20.393004905016586	19.643907678930308	31.435205400516626	28.52788201553648
5	15.31562899929551	38.32656036773007	32.7745078715095	13.583302761464921
6	33.920495537764666	37.43217053349718	14.362013046721675	14.28532088201648
7	29.464258263599607	32.0604747708996	19.442086192864007	19.03318077263678
8	27.655730268413148	36.20334174884919	17.14873394741136	18.992194035326303
9	27.501817808011182	14.763864145847148	18.214596597516085	39.51972144862559
10	17.01815355974435	29.120199784409383	30.390656608286186	23.47099004756008
11	37.577217937969124	22.46412717394277	20.51015567604068	19.448499212047423
12	23.624581857002873	25.22891177666095	28.125333029029285	23.02117333730689
13	30.872444105350816	20.89729455468655	24.57534967458643	23.654911665376204
14	22.845871571746123	20.62078025695459	26.24345141147723	30.28989675982206
15	25.90697538438034	28.236561188219284	22.03841587109077	23.81804755630961
16	24.873404172046214	27.691492281271252	23.397278050593062	24.037825496089475
17	24.56661665140431	27.21762674908025	25.065455234768375	23.150301364747065
18	25.044518613316637	25.835149569526088	25.686876793641307	23.433455023515975
19	26.779749571600885	25.599527699998966	25.650699820718394	21.97002290768176
20	25.54407394588355	25.827491670148245	25.602017460387817	23.02641692358039
21	25.901939278139242	25.36307591034222	25.637081585864195	23.09790322565434
22	24.885060777503128	25.51698837074418	25.977594042682416	23.620356809070273
23	24.41741964628427	25.19265935645352	26.75766237906036	23.632258618201845
24	24.17674280869492	25.877909318022564	26.326481148081328	23.618866725201183
25	24.690312474359715	25.941530240686266	26.65339423186648	22.714763053087538
26	24.352874494385308	26.05596490946797	26.536337769948027	23.05482282619869
27	24.889059483582198	26.21140517738128	25.54537541154136	23.35415992749516
28	25.266767451664222	25.91470873104269	25.626254900536903	23.19226891675618
29	24.965430997328223	25.83379151840489	25.84024226123056	23.36053522303632
30	24.582366272045935	26.393195409409977	25.518006909085077	23.506431409459015
31	24.64253548144327	25.909238802915663	26.115021271418776	23.333204444222293
32	24.965110346369052	25.920423862844384	25.834583714892258	23.27988207589431
33	25.47434179317447	25.62731116252005	25.886114210213112	23.012232834092362
34	25.29453205236419	25.501502815598347	25.470324225274275	23.73364090676319
35	25.124247531223386	26.407794458962808	25.415455187613816	23.052502822199987
36	24.978577686654223	25.845184058366023	26.030199661807547	23.14603859317221
37	25.293777579519077	25.527003997762986	25.73763395429592	23.44158446842201
38	24.85474983095093	25.660790895021705	25.836790547964195	23.64766872606317
39	24.571709343108786	25.619860743174616	26.277327242222565	23.531102671494036
40	24.83004084527365	25.973934849383646	26.061585732164026	23.134438573178677
41	25.4939769489684	25.511103482552343	25.92442256892345	23.070496999555804
42	25.566689269415647	25.535246613595792	25.491355155831652	23.40670896115691
43	25.310187363900173	24.828531899583435	25.816155715650503	24.04512502086589
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2146.0
1	1689.0
2	1232.0
3	1437.5
4	1643.0
5	1643.0
6	2500.0
7	3357.0
8	2889.5
9	2422.0
10	3405.5
11	4389.0
12	4389.0
13	8746.5
14	13104.0
15	26652.5
16	40201.0
17	37319.0
18	34437.0
19	34437.0
20	38191.5
21	41946.0
22	37126.0
23	32306.0
24	38459.0
25	44612.0
26	44612.0
27	52669.0
28	60726.0
29	72991.0
30	85256.0
31	100942.0
32	116628.0
33	116628.0
34	135019.0
35	153410.0
36	174076.5
37	194743.0
38	222156.5
39	249570.0
40	249570.0
41	276180.0
42	302790.0
43	333655.0
44	364520.0
45	401466.5
46	438413.0
47	438413.0
48	471254.5
49	504096.0
50	509810.5
51	515525.0
52	511773.0
53	508021.0
54	508021.0
55	485105.0
56	462189.0
57	404672.5
58	347156.0
59	301963.5
60	256771.0
61	256771.0
62	218998.5
63	181226.0
64	157397.0
65	133568.0
66	107438.0
67	81308.0
68	81308.0
69	65161.0
70	49014.0
71	40468.5
72	31923.0
73	25463.5
74	19004.0
75	19004.0
76	14900.5
77	10797.0
78	8437.5
79	6078.0
80	4794.5
81	3511.0
82	3511.0
83	2732.0
84	1953.0
85	1487.0
86	1021.0
87	748.0
88	475.0
89	475.0
90	319.5
91	164.0
92	101.5
93	39.0
94	28.5
95	18.0
96	18.0
97	10.5
98	3.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5301715.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.322568212662745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.33017858560603	35.358099042164646
2	12.227070650283157	11.327786284775762
3	4.300057242399862	5.975690847882662
4	2.1152461475012188	3.9193453581678916
5	1.197707002385095	2.774043215838369
6	0.7742029930972765	2.151784257491775
7	0.546335190470218	1.7715354389274338
8	0.40445367131874427	1.4988266222819526
9	0.3026267829146561	1.2616604815088555
>10	1.6296496668237237	13.449533338159695
>50	0.09654057354153515	3.057936792467764
>100	0.05799074921806548	5.388113279974449
>500	0.009399482085304327	2.9915185558886117
>1k	0.008132589514999709	6.897579501533382
>5k	2.8607098796454815E-4	0.8752076131571729
>10k+	1.2260185198480634E-4	1.301339369779598
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	25667	0.48412636288446287	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22588	0.42605081563230013	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20487	0.38642212944301985	No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC	9844	0.18567576718099707	No Hit
ATTCAGACTCGCTTTCGCTACGCATTCCCCTCTCGGGTTAAGC	8192	0.1545160386780504	No Hit
ATCCATGGCCAGGTTGAAGCGACGGTAAGACGTCGTGGAGGAC	6375	0.12024410968903458	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6226	0.11743369834100852	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.88618211276917E-5	0.0	0.0	3.77236422553834E-5	0.0
3	1.88618211276917E-5	0.0	0.0	5.65854633830751E-5	0.0
4	1.88618211276917E-5	0.0	0.0	7.54472845107668E-5	1.88618211276917E-5
5	3.77236422553834E-5	0.0	0.0	1.320327478938419E-4	1.88618211276917E-5
6	3.77236422553834E-5	0.0	0.0	1.508945690215336E-4	3.77236422553834E-5
7	3.77236422553834E-5	0.0	0.0	1.508945690215336E-4	5.65854633830751E-5
8	3.77236422553834E-5	0.0	0.0	1.508945690215336E-4	5.65854633830751E-5
9	3.77236422553834E-5	0.0	0.0	1.697563901492253E-4	5.65854633830751E-5
10	5.65854633830751E-5	0.0	0.0	2.829273169153755E-4	5.65854633830751E-5
11	7.54472845107668E-5	0.0	0.0	2.829273169153755E-4	5.65854633830751E-5
12	7.54472845107668E-5	0.0	0.0	2.829273169153755E-4	5.65854633830751E-5
13	7.54472845107668E-5	0.0	0.0	2.829273169153755E-4	7.54472845107668E-5
14	9.430910563845849E-5	0.0	0.0	2.829273169153755E-4	7.54472845107668E-5
15	9.430910563845849E-5	0.0	0.0	2.829273169153755E-4	7.54472845107668E-5
16	9.430910563845849E-5	0.0	0.0	3.017891380430672E-4	9.430910563845849E-5
17	1.131709267661502E-4	0.0	0.0	3.017891380430672E-4	9.430910563845849E-5
18	1.131709267661502E-4	0.0	0.0	3.206509591707589E-4	9.430910563845849E-5
19	1.131709267661502E-4	0.0	0.0	3.583746014261423E-4	1.131709267661502E-4
20	1.697563901492253E-4	0.0	0.0	4.1496006480921737E-4	1.320327478938419E-4
21	2.263418535323004E-4	0.0	0.0	7.167492028522846E-4	1.320327478938419E-4
22	2.263418535323004E-4	0.0	0.0	9.053674141292015E-4	1.320327478938419E-4
23	2.263418535323004E-4	0.0	0.0	9.808146986399684E-4	1.320327478938419E-4
24	2.263418535323004E-4	0.0	0.0	0.0011505710887891936	1.320327478938419E-4
25	2.263418535323004E-4	0.0	0.0	0.001320327478938419	1.320327478938419E-4
26	2.263418535323004E-4	0.0	0.0	0.0015655311535984111	1.320327478938419E-4
27	2.263418535323004E-4	0.0	0.0	0.0020182148606630117	1.320327478938419E-4
28	2.263418535323004E-4	0.0	0.0	0.0033385423396014306	1.320327478938419E-4
29	2.263418535323004E-4	0.0	0.0	0.0059414736552228856	1.320327478938419E-4
30	2.263418535323004E-4	0.0	0.0	0.009694976059633533	1.320327478938419E-4
31	2.452036746599921E-4	0.0	0.0	0.01599482431628256	1.320327478938419E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6895	0.0	30.802032	1
TACGCAT	1345	0.0	21.319704	19
CTACGCA	1450	0.0	19.775862	18
ACGCATT	1505	0.0	19.421928	20
CGCTACG	1470	0.0	19.380953	16
CGGGTTA	1550	0.0	18.141935	34
GCTACGC	1620	0.0	17.814816	17
CGCATTC	1645	0.0	17.431612	21
GCTTTCG	1620	0.0	17.243828	11
GTATCAA	12490	0.0	16.944754	2
CGACGCT	790	0.0	16.626581	29
CGCTTTC	1740	0.0	16.267242	10
TGAATCG	1360	0.0	16.051472	18
GAATCGC	1355	0.0	15.97417	19
CGCCCTA	1335	0.0	15.936329	23
AATCGCC	1335	0.0	15.936329	20
CTCGGGT	1835	0.0	15.929155	32
TCGAAAT	2420	0.0	15.82438	26
TCGGGTT	1810	0.0	15.740332	33
GTCGAAA	2485	0.0	15.484909	25
>>END_MODULE