FastQCFastQC Report
Thu 26 May 2016
SRR1041736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7625226
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT401890.5270532309468599No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT329770.43247242770247074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT266860.34996995498887506No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77850.1020953346169674No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC77590.10175436111664102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70850.030.2110081
GTATCAA163350.020.8953151
GTGCGAA19350.018.64341211
GTCGAAA19000.018.11052725
TCGAAAT18950.018.06068426
TGCGAAC19700.018.03045812
CCCCGTA20200.017.40099118
CCGTAGT20200.017.21782320
TAGTCGA21150.016.79432723
CCCGTAG20950.016.6897419
AGTCGAA21450.016.64568924
GTAGTCG21400.016.33878522
CGTAGTC21950.015.92938421
GGTGCGA23450.015.30490410
ACCCCGT23350.015.13276217
TCGTCTA10800.014.560184527
CGACGCT8850.014.005650529
TATCAAC245650.013.7818032
GCGAACC28350.013.63844813
CGTCTAT11150.013.60538128
Produced by FastQC (version 0.11.5)