##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041736_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7625226
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.964975595477434	31.0	31.0	34.0	31.0	34.0
2	32.24290283330619	33.0	31.0	34.0	31.0	34.0
3	32.3207815742117	34.0	31.0	34.0	31.0	34.0
4	35.99428462841626	37.0	35.0	37.0	35.0	37.0
5	35.87305727069597	37.0	35.0	37.0	35.0	37.0
6	35.999176680140366	37.0	35.0	37.0	35.0	37.0
7	35.954383909408065	37.0	35.0	37.0	35.0	37.0
8	35.93816209513003	37.0	35.0	37.0	35.0	37.0
9	37.56317897987548	39.0	37.0	39.0	35.0	39.0
10	37.41700639430228	39.0	37.0	39.0	35.0	39.0
11	37.56158637134165	39.0	37.0	39.0	35.0	39.0
12	37.38035803791258	39.0	37.0	39.0	35.0	39.0
13	37.42501127179706	39.0	37.0	39.0	35.0	39.0
14	38.27950738771546	40.0	38.0	40.0	35.0	40.0
15	38.28744918511268	40.0	38.0	40.0	35.0	40.0
16	38.25794501041674	40.0	38.0	40.0	35.0	40.0
17	38.231075905160054	40.0	38.0	40.0	35.0	40.0
18	38.16633985668097	40.0	38.0	40.0	34.0	40.0
19	38.16943051917412	40.0	38.0	40.0	34.0	40.0
20	38.09040191071058	40.0	38.0	40.0	34.0	40.0
21	38.01614365266026	40.0	38.0	40.0	34.0	40.0
22	37.927883711250004	40.0	38.0	40.0	34.0	40.0
23	37.840213653995306	40.0	38.0	40.0	34.0	40.0
24	37.75556029421292	40.0	37.0	40.0	33.0	40.0
25	37.700770573882004	40.0	37.0	40.0	33.0	40.0
26	37.52029041499885	39.0	37.0	40.0	33.0	40.0
27	37.41926088485771	39.0	37.0	40.0	33.0	40.0
28	37.306317609471506	39.0	37.0	40.0	32.0	40.0
29	37.21331748068844	39.0	37.0	40.0	32.0	40.0
30	37.0917595098165	39.0	36.0	40.0	32.0	40.0
31	36.96355268683184	39.0	36.0	40.0	31.0	40.0
32	37.06147778439616	39.0	36.0	40.0	32.0	40.0
33	37.2150032012166	39.0	37.0	40.0	32.0	40.0
34	37.22862850228964	39.0	37.0	40.0	33.0	40.0
35	37.26704559314045	39.0	37.0	40.0	33.0	40.0
36	37.240585787227815	39.0	37.0	40.0	33.0	40.0
37	37.18715012512416	39.0	37.0	40.0	32.0	40.0
38	37.12000890203123	39.0	36.0	40.0	32.0	40.0
39	37.03323193306008	39.0	36.0	40.0	32.0	40.0
40	36.98005160765071	39.0	36.0	40.0	32.0	40.0
41	36.90716117791132	39.0	36.0	40.0	31.0	40.0
42	36.79205416337824	39.0	36.0	40.0	31.0	40.0
43	36.29953577769367	38.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	5.0
14	10.0
15	14.0
16	35.0
17	69.0
18	156.0
19	383.0
20	717.0
21	1617.0
22	3175.0
23	5769.0
24	9823.0
25	16247.0
26	24802.0
27	36501.0
28	52106.0
29	71490.0
30	96330.0
31	127323.0
32	166584.0
33	226062.0
34	323351.0
35	490845.0
36	850987.0
37	1648217.0
38	3163105.0
39	309500.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.160625796533765	20.166195729805253	14.033144722530192	23.640033751130787
2	18.575357635301565	21.42489940626022	36.370082670336586	23.629660288101626
3	20.46500654537977	24.325154428209732	29.85318730225176	25.356651724158734
4	15.46411870284238	17.393530368804807	35.043512152951266	32.098838775401546
5	14.872306735564297	36.83131490135506	33.69375543754376	14.602622925536895
6	33.60674162313353	36.497816589305025	15.425169037612788	14.470272749948657
7	29.19020367396324	31.55474998380376	20.305929293112097	18.949117049120908
8	27.05914552565393	35.32593001177932	18.181900444655675	19.433024017911077
9	27.02470720212096	15.166934068577115	18.69455410239644	39.113804626905484
10	16.31391646621359	28.278466762821193	31.445795836084073	23.961820934881143
11	36.083664930062405	22.567304890373087	21.760181272004267	19.588848907560248
12	23.672295090007825	24.622221033186424	28.910893919734313	22.794589957071437
13	29.735249814235015	21.089945399651107	25.016032311698037	24.15877247441584
14	22.84500157765816	20.25950443960612	26.31270207597781	30.58279190675791
15	24.746610788978582	27.91685125135963	23.0894664630268	24.247071496634984
16	24.30915490242519	27.405613945081758	24.099246369878085	24.185984782614966
17	23.751282912794977	26.7840061396213	25.70281851318243	23.76189243440129
18	24.510604669291112	25.947099797435513	26.287260207107305	23.25503532616607
19	25.75791196221594	25.570166707190054	26.330734328398925	22.34118700219508
20	24.94184434664625	25.62236188147079	26.250762928206978	23.185030843675978
21	24.832955770753546	25.36913660001684	26.509653615512512	23.2882540137171
22	24.308158210655	25.29787313844862	26.54154775215843	23.852420898737954
23	23.954660491374288	25.105813257207068	26.975947991574284	23.963578259844365
24	23.753735299124248	25.52574835158984	26.844450249736862	23.87606609954905
25	24.145802367038037	25.4499473195942	26.939752343078094	23.464497970289667
26	23.941978900035224	25.633128775461866	26.887098166008457	23.53779415849445
27	24.21798383418406	25.85174524663269	26.33885212057977	23.591418798603478
28	24.251386122850654	25.589956284574384	26.513810869343413	23.644846723231545
29	24.272552708601687	25.57974019392999	26.427583916857024	23.720123180611303
30	24.124937411691143	25.93524441111647	26.398365110752124	23.54145306644026
31	24.24297981463107	25.427666012784407	26.699890599963854	23.62946357262067
32	24.29736508793313	25.571635516114537	26.473825169247444	23.65717422670489
33	24.47467130810287	25.513971651463187	26.47517594888335	23.5361810915506
34	24.400824316551404	25.38146410349018	26.358812184714264	23.858899395244155
35	24.351671675042812	25.887219605032037	26.160326264428097	23.600782455497058
36	24.31054502515729	25.552934431058173	26.562976625217406	23.573543918567136
37	24.62813561198055	25.30941377999813	26.300374572504477	23.76207603551685
38	24.24383224838188	25.539531549622268	26.376398548711865	23.84023765328398
39	24.16132977566829	25.53873157333304	26.597493634942754	23.70244501605592
40	24.325324914959896	25.523282850895175	26.684232047679636	23.467160186465293
41	24.59193996348436	25.389175350343713	26.480710211080954	23.53817447509097
42	24.60779523124954	25.419994109027062	26.262329798487283	23.709880861236112
43	24.52475506955466	25.025317282399236	26.373880590555608	24.076047057490495
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2966.0
1	2307.0
2	1648.0
3	2618.0
4	3588.0
5	3588.0
6	4609.5
7	5631.0
8	4656.0
9	3681.0
10	5141.5
11	6602.0
12	6602.0
13	11777.0
14	16952.0
15	35009.5
16	53067.0
17	57243.5
18	61420.0
19	61420.0
20	59748.5
21	58077.0
22	54966.5
23	51856.0
24	62322.5
25	72789.0
26	72789.0
27	86358.5
28	99928.0
29	118579.0
30	137230.0
31	161350.5
32	185471.0
33	185471.0
34	213514.5
35	241558.0
36	274092.5
37	306627.0
38	344605.0
39	382583.0
40	382583.0
41	419809.0
42	457035.0
43	497821.5
44	538608.0
45	579128.0
46	619648.0
47	619648.0
48	653549.0
49	687450.0
50	692860.5
51	698271.0
52	686456.5
53	674642.0
54	674642.0
55	643261.0
56	611880.0
57	550723.5
58	489567.0
59	431135.0
60	372703.0
61	372703.0
62	321828.5
63	270954.0
64	232717.5
65	194481.0
66	158221.5
67	121962.0
68	121962.0
69	98360.0
70	74758.0
71	62544.5
72	50331.0
73	40479.0
74	30627.0
75	30627.0
76	24113.5
77	17600.0
78	13973.5
79	10347.0
80	8249.0
81	6151.0
82	6151.0
83	4832.0
84	3513.0
85	2693.0
86	1873.0
87	1311.0
88	749.0
89	749.0
90	504.0
91	259.0
92	174.0
93	89.0
94	61.0
95	33.0
96	33.0
97	24.5
98	16.0
99	10.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7625226.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.89716424511357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.7522792993046	33.850204764516725
2	13.074324601300052	12.001488472395948
3	4.779773809027119	6.581341907022292
4	2.3967167554935163	4.400100103036064
5	1.3997647100449053	3.21226154007224
6	0.9341374510699451	2.5724556011561406
7	0.6380341456585858	2.0498770584097983
8	0.46882639328799197	1.7214241580146543
9	0.3606450349769664	1.4897325964070298
>10	2.0043983323383743	16.666526672968335
>50	0.12253651635768834	3.8505808894458315
>100	0.058744119108419195	5.081577043928372
>500	0.005828315836952525	1.8923118641970156
>1k	0.0038469724494341164	3.109601735230818
>5k	5.7417498195963456E-5	0.20481554283233622
>10k+	8.612624729394518E-5	1.315700050366343
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	40189	0.5270532309468599	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32977	0.43247242770247074	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26686	0.34996995498887506	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7785	0.1020953346169674	No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC	7759	0.10175436111664102	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.3114365397169868E-5	0.0	0.0	1.3114365397169868E-5	0.0
3	1.3114365397169868E-5	0.0	0.0	1.3114365397169868E-5	0.0
4	2.6228730794339736E-5	0.0	0.0	2.6228730794339736E-5	0.0
5	3.9343096191509604E-5	0.0	0.0	6.557182698584933E-5	0.0
6	6.557182698584933E-5	0.0	0.0	6.557182698584933E-5	0.0
7	6.557182698584933E-5	0.0	0.0	6.557182698584933E-5	0.0
8	6.557182698584933E-5	0.0	0.0	6.557182698584933E-5	0.0
9	6.557182698584933E-5	0.0	0.0	9.180055778018907E-5	1.3114365397169868E-5
10	9.180055778018907E-5	0.0	0.0	1.3114365397169867E-4	1.3114365397169868E-5
11	1.7048675016320827E-4	0.0	0.0	1.5737238476603842E-4	1.3114365397169868E-5
12	1.7048675016320827E-4	0.0	0.0	1.5737238476603842E-4	1.3114365397169868E-5
13	1.7048675016320827E-4	0.0	0.0	1.7048675016320827E-4	1.3114365397169868E-5
14	1.7048675016320827E-4	0.0	0.0	2.098298463547179E-4	1.3114365397169868E-5
15	1.7048675016320827E-4	0.0	0.0	2.098298463547179E-4	2.6228730794339736E-5
16	2.098298463547179E-4	0.0	0.0	2.2294421175188774E-4	3.9343096191509604E-5
17	2.098298463547179E-4	1.3114365397169868E-5	0.0	2.4917294254622746E-4	3.9343096191509604E-5
18	2.098298463547179E-4	2.6228730794339736E-5	0.0	2.4917294254622746E-4	5.245746158867947E-5
19	2.098298463547179E-4	2.6228730794339736E-5	0.0	3.0163040413490696E-4	5.245746158867947E-5
20	2.2294421175188774E-4	2.6228730794339736E-5	0.0	3.672022311207563E-4	5.245746158867947E-5
21	2.4917294254622746E-4	2.6228730794339736E-5	0.0	4.065453273122659E-4	7.868619238301921E-5
22	2.4917294254622746E-4	2.6228730794339736E-5	0.0	5.508033466811344E-4	7.868619238301921E-5
23	2.4917294254622746E-4	2.6228730794339736E-5	0.0	6.163751736669837E-4	1.5737238476603842E-4
24	2.4917294254622746E-4	3.9343096191509604E-5	0.0	7.868619238301921E-4	1.5737238476603842E-4
25	2.4917294254622746E-4	5.245746158867947E-5	0.0	8.130906546245318E-4	1.7048675016320827E-4
26	2.754016733405672E-4	5.245746158867947E-5	0.0	9.573486739934004E-4	1.7048675016320827E-4
27	2.754016733405672E-4	5.245746158867947E-5	0.0	0.001495037655277365	1.7048675016320827E-4
28	2.754016733405672E-4	5.245746158867947E-5	0.0	0.0030031896759518998	1.8360111556037814E-4
29	2.754016733405672E-4	5.245746158867947E-5	0.0	0.006137523005875498	1.8360111556037814E-4
30	2.885160387377371E-4	6.557182698584933E-5	0.0	0.00986200277867174	1.8360111556037814E-4
31	2.885160387377371E-4	6.557182698584933E-5	0.0	0.016327384919476485	1.8360111556037814E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7085	0.0	30.211008	1
GTATCAA	16335	0.0	20.895315	1
GTGCGAA	1935	0.0	18.643412	11
GTCGAAA	1900	0.0	18.110527	25
TCGAAAT	1895	0.0	18.060684	26
TGCGAAC	1970	0.0	18.030458	12
CCCCGTA	2020	0.0	17.400991	18
CCGTAGT	2020	0.0	17.217823	20
TAGTCGA	2115	0.0	16.794327	23
CCCGTAG	2095	0.0	16.68974	19
AGTCGAA	2145	0.0	16.645689	24
GTAGTCG	2140	0.0	16.338785	22
CGTAGTC	2195	0.0	15.929384	21
GGTGCGA	2345	0.0	15.304904	10
ACCCCGT	2335	0.0	15.132762	17
TCGTCTA	1080	0.0	14.5601845	27
CGACGCT	885	0.0	14.0056505	29
TATCAAC	24565	0.0	13.781803	2
GCGAACC	2835	0.0	13.638448	13
CGTCTAT	1115	0.0	13.605381	28
>>END_MODULE