FastQCFastQC Report
Thu 26 May 2016
SRR1041735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10471138
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1358861.2977195028849777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1323971.2643993422682425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT801880.765800240623321No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354390.338444589308249No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT281640.26896790014609684No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263000.2511665876240004No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206250.19696999504733867No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG146900.14029038677553482No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133800.12777980769616445No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131410.1254973432687068No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT122990.11745619244059241No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT113030.10794433231612456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA400650.014.6189951
GTATCAA665900.014.3549341
TATACCG4850.012.2061855
CGTTTCG1257.2272937E-411.84000129
AACGCAG915200.010.9884185
CGATGCG5650.010.8053133
TATCAAC928100.010.7380131
ATACGTA8650.010.69364125
GTATTAT20000.010.6375011
GTATAAA31000.010.5032251
ATCAACG952700.010.478222
GTATAGA21400.010.4602811
TCAACGC959150.010.4579073
CAACGCA966300.010.4073274
ATAGCGT6250.010.3600016
GTATAAG20600.010.327671
ACGCAGA975850.010.3244346
TATACTG28350.010.3104065
CGCAGAG982200.010.2897077
AGAGTAC991450.010.22169510
Produced by FastQC (version 0.11.5)