##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041735_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10471138
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.98537074002845	34.0	33.0	34.0	31.0	34.0
2	33.14568311486297	34.0	33.0	34.0	31.0	34.0
3	33.19935836964425	34.0	33.0	34.0	31.0	34.0
4	36.52127686599107	37.0	37.0	37.0	35.0	37.0
5	36.52195024074747	37.0	37.0	37.0	35.0	37.0
6	36.59100749125835	37.0	37.0	37.0	35.0	37.0
7	36.55058485524687	37.0	37.0	37.0	35.0	37.0
8	36.541529488007896	37.0	37.0	37.0	35.0	37.0
9	38.32161719194227	39.0	39.0	39.0	37.0	39.0
10	38.32913967899191	39.0	39.0	39.0	37.0	39.0
11	38.38125540891544	39.0	39.0	39.0	37.0	39.0
12	38.30479733912398	39.0	39.0	39.0	37.0	39.0
13	38.308928217735264	39.0	39.0	39.0	37.0	39.0
14	39.17312397181663	40.0	40.0	40.0	38.0	40.0
15	39.18499985388408	40.0	40.0	40.0	38.0	40.0
16	39.18562643334469	40.0	40.0	40.0	38.0	40.0
17	39.22879576221801	40.0	40.0	40.0	38.0	40.0
18	39.21702712732847	40.0	40.0	40.0	38.0	40.0
19	39.24556977474655	40.0	40.0	40.0	38.0	40.0
20	39.2051690083733	40.0	40.0	40.0	38.0	40.0
21	39.15716744445542	40.0	40.0	40.0	37.0	40.0
22	39.10216807380439	40.0	40.0	40.0	37.0	40.0
23	39.04519919420411	40.0	40.0	40.0	37.0	40.0
24	39.007842796074314	40.0	39.0	40.0	37.0	40.0
25	38.972048501318575	40.0	39.0	40.0	37.0	40.0
26	38.8764263253908	40.0	39.0	40.0	36.0	40.0
27	38.80453194294641	40.0	39.0	40.0	36.0	40.0
28	38.7454196477976	40.0	39.0	40.0	36.0	40.0
29	38.6613218162152	40.0	39.0	40.0	35.0	40.0
30	38.56548084840444	40.0	38.0	40.0	35.0	40.0
31	38.43935453816004	40.0	38.0	40.0	35.0	40.0
32	38.459543174772406	40.0	38.0	40.0	35.0	40.0
33	38.501500600985295	40.0	39.0	40.0	35.0	40.0
34	38.48134949611016	40.0	39.0	40.0	35.0	40.0
35	38.489574963103344	40.0	39.0	40.0	35.0	40.0
36	38.47071321187821	40.0	39.0	40.0	35.0	40.0
37	38.418416699311955	40.0	39.0	40.0	35.0	40.0
38	38.37719797026837	40.0	39.0	40.0	35.0	40.0
39	38.330124672218055	40.0	39.0	40.0	35.0	40.0
40	38.27616119661492	40.0	38.0	40.0	35.0	40.0
41	38.22081095674606	40.0	38.0	40.0	35.0	40.0
42	38.12378979247528	40.0	38.0	40.0	35.0	40.0
43	37.84253211064547	40.0	38.0	40.0	35.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	3.0
12	3.0
13	19.0
14	22.0
15	29.0
16	47.0
17	89.0
18	121.0
19	179.0
20	322.0
21	613.0
22	1203.0
23	2414.0
24	4560.0
25	7655.0
26	12512.0
27	19432.0
28	30025.0
29	43012.0
30	57822.0
31	75459.0
32	98261.0
33	132962.0
34	194386.0
35	300025.0
36	528841.0
37	1156744.0
38	5060980.0
39	2743393.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.965928058631256	19.07496587285928	14.69359872823756	22.2655073402719
2	15.268168560093468	22.78716028764018	39.29911915973221	22.645551992534145
3	16.905249458081826	27.1620238411527	32.427965327168835	23.50476137359664
4	11.797609772691372	17.489951903985983	38.63843643355669	32.074001889765945
5	12.966747262809447	38.47136767751509	34.585047012082164	13.976838047593299
6	29.839679316612962	39.52505448786942	16.299670580217736	14.335595615299884
7	25.840801639707166	33.87718698769895	22.689253068768647	17.59275830382524
8	26.4496179880353	35.26378890240965	20.031815071103065	18.25477803845198
9	26.486414370625237	15.092858101956065	20.317228175199293	38.10349935221941
10	14.709232177056592	30.000970286133178	33.032789750264016	22.257007786546218
11	32.99687197322774	24.193750478696778	23.96252441711684	18.846853130958642
12	22.384682543578357	27.16427765539906	30.788153111915822	19.662886689106763
13	28.72294300772275	23.008263285232225	27.0165382215381	21.252255485506925
14	22.084180344103956	21.7848146018131	29.7499660495354	26.381039004547546
15	22.976031831497206	30.097082093655914	25.918500930844385	21.008385144002496
16	21.60610432218542	28.62972486849089	27.146075240341595	22.618095568982092
17	21.46630098848855	28.093183377012128	28.446965363268063	21.993550271231264
18	22.34033206323897	26.204592089226598	30.35734033874828	21.09773550878615
19	23.463180410763375	26.69112946462935	30.17447578286142	19.671214341745856
20	22.5634310234475	26.660645671941293	30.39867300001203	20.377250304599176
21	21.897476663949995	27.05998144614272	30.422385799900642	20.620156090006645
22	21.541966116767824	26.645231874510678	30.389161139887566	21.42364086883393
23	21.327395360466074	26.31965121651534	30.528219568875897	21.824733854142693
24	20.94928937045811	26.735556345451663	30.604390850354562	21.710763433735664
25	21.54371377781479	26.60627717827804	30.6480537263476	21.201955317559563
26	21.484665754572234	26.54480343970254	30.74680135053134	21.223729455193887
27	21.56077973568871	27.06975115789707	30.594926740531925	20.774542365882294
28	21.89602505477437	26.48389315468863	30.652618655202517	20.96746313533448
29	21.26416441078324	26.926146900174558	30.929876007746245	20.87981268129596
30	21.617134641908073	26.944521216318606	30.64698411958662	20.7913600221867
31	21.50751904902791	26.390283462981767	30.915999770034546	21.186197717955775
32	21.234196321354947	26.951530960627203	30.36889591179106	21.445376806226793
33	21.389470752844627	26.80047765581926	30.38625792153632	21.42379366979979
34	21.567693979393642	27.096959279879606	30.39926510375472	20.936081636972027
35	21.52848143153113	27.12636391574631	30.320190603924807	21.024964048797752
36	21.61617963587148	27.12752902311096	30.099546009230327	21.156745331787242
37	21.35888190949255	26.754455914915837	30.321279310806524	21.565382864785086
38	21.531470600425664	27.080619126593497	30.168831697185155	21.219078575795677
39	21.51080426979379	27.080542726110572	29.990264668462967	21.418388335632667
40	21.645737072704037	26.88909266595474	30.54715733858154	20.918012922759686
41	21.786113410022864	26.83507752452503	30.22074582533436	21.15806324011774
42	21.64495396775403	26.936470515430127	30.24607258542481	21.17250293139103
43	21.586507598314526	26.597701224069436	30.438601802401994	21.37718937521404
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19651.0
1	16173.5
2	12696.0
3	23580.0
4	34464.0
5	34464.0
6	33654.5
7	32845.0
8	24140.5
9	15436.0
10	23243.0
11	31050.0
12	31050.0
13	49090.5
14	67131.0
15	131376.0
16	195621.0
17	197889.0
18	200157.0
19	200157.0
20	184393.5
21	168630.0
22	152131.5
23	135633.0
24	160785.0
25	185937.0
26	185937.0
27	214083.5
28	242230.0
29	278228.0
30	314226.0
31	355114.5
32	396003.0
33	396003.0
34	439211.5
35	482420.0
36	531731.0
37	581042.0
38	623445.0
39	665848.0
40	665848.0
41	693837.5
42	721827.0
43	747806.5
44	773786.0
45	786723.0
46	799660.0
47	799660.0
48	816870.0
49	834080.0
50	815788.0
51	797496.0
52	780116.0
53	762736.0
54	762736.0
55	677403.0
56	592070.0
57	515333.5
58	438597.0
59	388123.0
60	337649.0
61	337649.0
62	287072.0
63	236495.0
64	193022.0
65	149549.0
66	123803.5
67	98058.0
68	98058.0
69	77852.0
70	57646.0
71	47741.0
72	37836.0
73	29998.5
74	22161.0
75	22161.0
76	17116.5
77	12072.0
78	9312.5
79	6553.0
80	5962.5
81	5372.0
82	5372.0
83	4412.0
84	3452.0
85	2700.0
86	1948.0
87	1285.5
88	623.0
89	623.0
90	431.5
91	240.0
92	168.5
93	97.0
94	91.5
95	86.0
96	86.0
97	48.5
98	11.0
99	14.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.0471138E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.459222400669002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	52.02469509036669	13.76532977723005
2	18.438228488216637	9.7572237648815
3	9.631911409728211	7.6455865840062005
4	5.454396424628856	5.772763522426748
5	3.3311678010578354	4.407005485106839
6	2.2575651397708767	3.5840050870317013
7	1.593128500928176	2.950705892323211
8	1.1474813355733435	2.4289171086841437
9	0.8556059792171069	2.037480200230285
>10	4.696721838274414	22.52779783441318
>50	0.35233637598688156	6.43260493142577
>100	0.20123887707252375	9.767277228246229
>500	0.011553977343657783	2.048031414086379
>1k	0.0033914878662889736	1.5661717533475987
>5k	1.4431849214434524E-4	0.30858698241675797
>10k+	4.329554764330357E-4	5.000512434143444
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	135886	1.2977195028849777	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	132397	1.2643993422682425	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	80188	0.765800240623321	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35439	0.338444589308249	No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	28164	0.26896790014609684	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26300	0.2511665876240004	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20625	0.19696999504733867	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	14690	0.14029038677553482	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13380	0.12777980769616445	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13141	0.1254973432687068	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12299	0.11745619244059241	No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT	11303	0.10794433231612456	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.8650181097794718E-5	0.0
3	0.0	0.0	0.0	4.775030182965787E-5	0.0
4	0.0	0.0	0.0	9.550060365931573E-5	0.0
5	7.640048292745259E-5	0.0	0.0	1.432509054889736E-4	0.0
6	4.0110253536912606E-4	0.0	0.0	1.432509054889736E-4	0.0
7	4.1065259573505765E-4	0.0	0.0	1.6235102622083673E-4	0.0
8	4.584028975647155E-4	0.0	0.0	1.7190108658676832E-4	0.0
9	4.6795295793064707E-4	0.0	0.0	2.3875150914828933E-4	0.0
10	6.685042256152101E-4	0.0	0.0	3.6290229390539976E-4	0.0
11	7.735548896404574E-4	0.0	0.0	4.8705307866251025E-4	0.0
12	7.83104950006389E-4	0.0	0.0	5.539035012240313E-4	0.0
13	8.213051914701153E-4	0.0	0.0	6.016538030536891E-4	0.0
14	9.741061573250205E-4	0.0	0.0	6.398540445174154E-4	0.0
15	0.0010123063987887467	0.0	0.0	6.876043463470733E-4	0.0
16	0.0011746574250095835	0.0	0.0	7.353546481767312E-4	0.0
17	0.001184207485375515	0.0	0.0	8.213051914701153E-4	0.0
18	0.001184207485375515	0.0	0.0	9.16805795129431E-4	0.0
19	0.0011937575457414465	0.0	0.0	0.0010123063987887467	0.0
20	0.0013561085719622833	0.0	0.0	0.0012224077268392414	0.0
21	0.0017572111073314094	0.0	0.0	0.0019195621335522462	0.0
22	0.001766761167697341	0.0	0.0	0.002769517506120156	0.0
23	0.0017954113487951358	0.0	0.0	0.003714973482347382	0.0
24	0.0017954113487951358	0.0	0.0	0.005815986762852328	0.0
25	0.0018240615298929304	0.0	0.0	0.007238945757376132	0.0
26	0.0019864125561137673	0.0	0.0	0.009464109822638189	0.0
27	0.0020055126768456304	0.0	0.0	0.012548779320834086	0.0
28	0.0020628130390412196	0.0	0.0	0.024572305321541938	0.0
29	0.0020628130390412196	0.0	0.0	0.05458814505166487	0.0
30	0.0020628130390412196	0.0	0.0	0.09084017420074113	0.0
31	0.002101013280504946	0.0	0.0	0.12268007546075699	9.550060365931573E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	40065	0.0	14.618995	1
GTATCAA	66590	0.0	14.354934	1
TATACCG	485	0.0	12.206185	5
CGTTTCG	125	7.2272937E-4	11.840001	29
AACGCAG	91520	0.0	10.988418	5
CGATGCG	565	0.0	10.80531	33
TATCAAC	92810	0.0	10.738013	1
ATACGTA	865	0.0	10.693641	25
GTATTAT	2000	0.0	10.637501	1
GTATAAA	3100	0.0	10.503225	1
ATCAACG	95270	0.0	10.47822	2
GTATAGA	2140	0.0	10.460281	1
TCAACGC	95915	0.0	10.457907	3
CAACGCA	96630	0.0	10.407327	4
ATAGCGT	625	0.0	10.360001	6
GTATAAG	2060	0.0	10.32767	1
ACGCAGA	97585	0.0	10.324434	6
TATACTG	2835	0.0	10.310406	5
CGCAGAG	98220	0.0	10.289707	7
AGAGTAC	99145	0.0	10.221695	10
>>END_MODULE