FastQCFastQC Report
Thu 26 May 2016
SRR1041734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7394654
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT354630.4795761911240201No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT306270.4141775937048576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT214310.2898174816563425No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC97490.13183848764255907No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74290.10046447068382104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65800.027.5531921
GTATCAA148150.020.7165051
TCGAAAT23700.018.10970526
GTCGAAA23950.017.84342225
TAGTCGA25600.017.05468823
AGTCGAA26150.016.90822224
TGCGAAC25250.016.85148412
CCCGTAG25700.016.77237319
CCGTAGT25800.016.63565820
GTGCGAA26000.016.57884811
TACGCAT10100.016.4851519
GTAGTCG26800.016.15298522
TCGCGAA1751.3278623E-1015.85714234
CCCCGTA27300.015.78937818
CGTAGTC27350.015.76051121
ACCCCGT28300.015.16607817
CGAAATG28650.015.10994827
ACGCATT11450.014.8646320
CGGGTTA11300.014.57079734
CTACGCA11750.014.48510618
Produced by FastQC (version 0.11.5)