##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041734_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7394654
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89277442866157	34.0	31.0	34.0	31.0	34.0
2	33.09722050551655	34.0	33.0	34.0	31.0	34.0
3	33.14996874769259	34.0	33.0	34.0	31.0	34.0
4	36.49867133742836	37.0	37.0	37.0	35.0	37.0
5	36.47071844064644	37.0	37.0	37.0	35.0	37.0
6	36.529593000565	37.0	37.0	37.0	35.0	37.0
7	36.490391572073555	37.0	37.0	37.0	35.0	37.0
8	36.47595533205475	37.0	37.0	37.0	35.0	37.0
9	38.24508421895061	39.0	39.0	39.0	37.0	39.0
10	38.22654055213401	39.0	39.0	39.0	37.0	39.0
11	38.29006103598627	39.0	39.0	39.0	37.0	39.0
12	38.19562605633746	39.0	39.0	39.0	37.0	39.0
13	38.213897364230974	39.0	39.0	39.0	37.0	39.0
14	39.123932505834624	40.0	40.0	40.0	37.0	40.0
15	39.147121690886415	40.0	40.0	40.0	38.0	40.0
16	39.13497670073542	40.0	40.0	40.0	37.0	40.0
17	39.12935980506999	40.0	40.0	40.0	37.0	40.0
18	39.09156412186425	40.0	40.0	40.0	37.0	40.0
19	39.095164696008766	40.0	40.0	40.0	37.0	40.0
20	39.047128641853966	40.0	40.0	40.0	37.0	40.0
21	39.000008113969905	40.0	39.0	40.0	37.0	40.0
22	38.94568968338478	40.0	39.0	40.0	37.0	40.0
23	38.88870784217896	40.0	39.0	40.0	37.0	40.0
24	38.839841323204574	40.0	39.0	40.0	36.0	40.0
25	38.81131422781918	40.0	39.0	40.0	36.0	40.0
26	38.69840022264734	40.0	39.0	40.0	36.0	40.0
27	38.637339894469704	40.0	39.0	40.0	36.0	40.0
28	38.566580667601215	40.0	39.0	40.0	36.0	40.0
29	38.50467932644313	40.0	38.0	40.0	35.0	40.0
30	38.42231374179238	40.0	38.0	40.0	35.0	40.0
31	38.33248655041872	40.0	38.0	40.0	35.0	40.0
32	38.40296138264211	40.0	38.0	40.0	35.0	40.0
33	38.489921232284836	40.0	39.0	40.0	35.0	40.0
34	38.4902173921863	40.0	39.0	40.0	35.0	40.0
35	38.520441524376935	40.0	39.0	40.0	35.0	40.0
36	38.50274590264805	40.0	39.0	40.0	35.0	40.0
37	38.46359139994921	40.0	39.0	40.0	35.0	40.0
38	38.423620361412446	40.0	39.0	40.0	35.0	40.0
39	38.36963284556654	40.0	38.0	40.0	35.0	40.0
40	38.33323817449742	40.0	38.0	40.0	35.0	40.0
41	38.28200318770831	40.0	38.0	40.0	35.0	40.0
42	38.197294153316705	40.0	38.0	40.0	35.0	40.0
43	37.88140175321252	40.0	38.0	40.0	34.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	1.0
13	1.0
14	6.0
15	5.0
16	8.0
17	24.0
18	47.0
19	73.0
20	211.0
21	459.0
22	1050.0
23	2087.0
24	3812.0
25	6480.0
26	10450.0
27	15845.0
28	23196.0
29	32742.0
30	45184.0
31	60227.0
32	79092.0
33	105574.0
34	150058.0
35	220233.0
36	366452.0
37	797013.0
38	3831390.0
39	1642931.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.86857045644056	18.187301258449683	13.033550995083745	24.910577290026012
2	17.100881258271176	20.22888427234053	37.13485445025555	25.53538001913274
3	18.416615571195084	23.51984284863092	30.18851186275923	27.87502971741477
4	13.61664791888843	16.534255693369833	35.83449611029806	34.01460027744368
5	14.48004193299646	36.75280817736705	34.09273780761074	14.674412082025745
6	33.324777602846595	36.44601897533001	15.851086474093313	14.378116947730076
7	28.98141008355496	31.482825295138895	20.722659910795016	18.813104710511134
8	27.394790885415325	34.39737680762345	18.592120740199608	19.61571156676161
9	27.064917439003906	14.526548503824518	18.761499861927277	39.647034195244295
10	15.795654536371817	28.116230996068243	31.99710223088193	24.09101223667801
11	35.8028921975254	22.18443215869194	22.33923318116034	19.67344246262232
12	23.2518384227308	24.42601100741157	29.440228035010158	22.881922534847472
13	29.53654085776021	21.207875311001708	25.180123370207717	24.07546046103036
14	22.79837569141166	20.141321013802674	26.6299004659312	30.43040282885447
15	24.693704397798736	28.04051413358894	23.1205949595478	24.14518650906452
16	24.299324890657495	27.322495413578512	24.217603690449884	24.16057600531411
17	23.617886651626975	26.744036975901782	25.919860482992174	23.718215889479076
18	24.314660293774395	26.11205338343079	26.47776353024766	23.095522792547158
19	25.504546933500876	25.58614642415994	26.404061636960975	22.50524500537821
20	24.68066795282105	25.809280596495793	26.124684129913312	23.38536732076984
21	24.654797912113263	25.510524224662845	26.52807014364702	23.30660771957687
22	24.212573029109947	25.42970908442775	26.440263466012066	23.91745442045023
23	23.826943086180908	25.269701597938187	26.976867342271866	23.926487973609042
24	23.61932011964319	25.65035497265998	26.850803296543692	23.879521611153137
25	23.856802495424397	25.641605408447777	26.805297448670352	23.696294647457474
26	23.775919738773442	25.824264394250225	26.874293239413234	23.525522627563102
27	24.064709450908726	26.04248961479469	26.28045882877008	23.61234210552651
28	24.028832180653755	25.65602122830899	26.522904249475367	23.792242341561888
29	24.066819083083534	25.612286930531162	26.452231571619173	23.868662414766124
30	23.804332156717543	26.20095544700266	26.433380114877586	23.561332281402215
31	23.911044384226766	25.50364087352836	26.797102339068196	23.788212403176672
32	24.075717403410625	25.603997157946807	26.49504628614131	23.825239152501254
33	24.326885341761763	25.615262052828975	26.516250794154807	23.541601811254456
34	24.25757851550593	25.45378052847368	26.205336990750343	24.083303965270044
35	24.14041279010485	26.045572923357874	26.119666991856548	23.694347294680725
36	24.128363544798713	25.72304261970878	26.545190620142606	23.6034032153499
37	24.5569028652321	25.273393454244108	26.293576413446797	23.876127267077
38	24.092188762313963	25.639698625520545	26.404697231270053	23.863415380895443
39	23.978430904272194	25.562480678609166	26.71070208288312	23.748386334235516
40	24.166472156777044	25.514527116481716	26.810436296275657	23.508564430465576
41	24.51676576077799	25.43621378363342	26.4810632113416	23.565957244246995
42	24.4606576588979	25.50955054827447	26.376176627060577	23.653615165767054
43	24.19936078145103	25.125178270680415	26.440669164507224	24.23479178336133
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2376.0
1	2090.0
2	1804.0
3	3357.5
4	4911.0
5	4911.0
6	5458.5
7	6006.0
8	4682.5
9	3359.0
10	4884.0
11	6409.0
12	6409.0
13	10916.0
14	15423.0
15	31209.5
16	46996.0
17	50504.5
18	54013.0
19	54013.0
20	51778.0
21	49543.0
22	48856.0
23	48169.0
24	59225.0
25	70281.0
26	70281.0
27	84399.0
28	98517.0
29	116139.5
30	133762.0
31	158004.5
32	182247.0
33	182247.0
34	209724.5
35	237202.0
36	269088.5
37	300975.0
38	336780.5
39	372586.0
40	372586.0
41	409491.5
42	446397.0
43	484776.0
44	523155.0
45	565102.0
46	607049.0
47	607049.0
48	637835.5
49	668622.0
50	679089.5
51	689557.0
52	674755.0
53	659953.0
54	659953.0
55	629777.0
56	599601.0
57	538039.0
58	476477.0
59	414356.0
60	352235.0
61	352235.0
62	305311.5
63	258388.0
64	222200.0
65	186012.0
66	150266.5
67	114521.0
68	114521.0
69	91389.5
70	68258.0
71	56650.5
72	45043.0
73	36261.0
74	27479.0
75	27479.0
76	21875.0
77	16271.0
78	12909.0
79	9547.0
80	7571.0
81	5595.0
82	5595.0
83	4378.5
84	3162.0
85	2443.0
86	1724.0
87	1205.0
88	686.0
89	686.0
90	455.5
91	225.0
92	151.5
93	78.0
94	53.0
95	28.0
96	28.0
97	17.5
98	7.0
99	6.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7394654.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.41363772013527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.38696035259734	31.9652791767438
2	15.40501006218076	13.99195092077831
3	5.533244078809574	7.538542260364246
4	2.6780390007823063	4.864779719276828
5	1.5292309867085474	3.4723971010393484
6	0.934479372220173	2.5462864600167823
7	0.6464347217126358	2.0549866583101877
8	0.4773226286091779	1.7341565545023905
9	0.3589565207727512	1.467136925248854
>10	1.888556988370091	15.1801998337636
>50	0.09974052803657545	3.086029930117831
>100	0.050636007180472085	4.395653661872733
>500	0.006293947878163311	1.9823243434229243
>1k	0.004885018089825921	4.137449738256872
>5k	1.198777437683561E-4	0.39164743362106735
>10k+	8.990830782626706E-5	1.1911792826643535
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	35463	0.4795761911240201	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30627	0.4141775937048576	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21431	0.2898174816563425	No Hit
GTCATAGAGGGTGCGAACCCCGTAGTCGAAATGCAGCAATGGC	9749	0.13183848764255907	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7429	0.10046447068382104	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.352328317186984E-5	0.0
4	0.0	0.0	0.0	2.704656634373968E-5	0.0
5	0.0	1.352328317186984E-5	0.0	5.409313268747936E-5	0.0
6	1.352328317186984E-5	1.352328317186984E-5	0.0	5.409313268747936E-5	0.0
7	1.352328317186984E-5	1.352328317186984E-5	0.0	6.76164158593492E-5	0.0
8	1.352328317186984E-5	1.352328317186984E-5	0.0	6.76164158593492E-5	0.0
9	1.352328317186984E-5	1.352328317186984E-5	0.0	9.466298220308888E-5	0.0
10	2.704656634373968E-5	1.352328317186984E-5	0.0	1.6227939806243808E-4	1.352328317186984E-5
11	4.056984951560952E-5	1.352328317186984E-5	0.0	1.7580268123430792E-4	2.704656634373968E-5
12	4.056984951560952E-5	1.352328317186984E-5	0.0	1.7580268123430792E-4	2.704656634373968E-5
13	5.409313268747936E-5	1.352328317186984E-5	0.0	2.028492475780476E-4	2.704656634373968E-5
14	5.409313268747936E-5	1.352328317186984E-5	0.0	2.028492475780476E-4	2.704656634373968E-5
15	6.76164158593492E-5	2.704656634373968E-5	0.0	2.1637253074991745E-4	5.409313268747936E-5
16	6.76164158593492E-5	2.704656634373968E-5	0.0	2.4341909709365712E-4	5.409313268747936E-5
17	9.466298220308888E-5	2.704656634373968E-5	0.0	2.56942380265527E-4	5.409313268747936E-5
18	9.466298220308888E-5	2.704656634373968E-5	0.0	2.975122297811365E-4	6.76164158593492E-5
19	9.466298220308888E-5	2.704656634373968E-5	0.0	3.38082079296746E-4	6.76164158593492E-5
20	1.0818626537495873E-4	2.704656634373968E-5	0.0	3.5160536246861584E-4	6.76164158593492E-5
21	1.2170954854682856E-4	2.704656634373968E-5	0.0	4.8683819418731425E-4	8.113969903121904E-5
22	1.2170954854682856E-4	2.704656634373968E-5	0.0	7.302572912809713E-4	8.113969903121904E-5
23	1.2170954854682856E-4	2.704656634373968E-5	0.0	9.195832556871492E-4	1.0818626537495873E-4
24	1.352328317186984E-4	2.704656634373968E-5	0.0	0.0013117584676713744	1.0818626537495873E-4
25	1.352328317186984E-4	2.704656634373968E-5	0.0	0.0014199447330463333	1.0818626537495873E-4
26	1.6227939806243808E-4	2.704656634373968E-5	0.0	0.0017309802459993396	1.0818626537495873E-4
27	1.6227939806243808E-4	2.704656634373968E-5	0.0	0.0025694238026552694	1.0818626537495873E-4
28	1.6227939806243808E-4	2.704656634373968E-5	0.0	0.005842058330247771	1.0818626537495873E-4
29	1.6227939806243808E-4	2.704656634373968E-5	0.0	0.012468467084463992	1.0818626537495873E-4
30	1.6227939806243808E-4	2.704656634373968E-5	0.0	0.02113689159763256	1.0818626537495873E-4
31	1.6227939806243808E-4	2.704656634373968E-5	0.0	0.034349139256549394	1.2170954854682856E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6580	0.0	27.553192	1
GTATCAA	14815	0.0	20.716505	1
TCGAAAT	2370	0.0	18.109705	26
GTCGAAA	2395	0.0	17.843422	25
TAGTCGA	2560	0.0	17.054688	23
AGTCGAA	2615	0.0	16.908222	24
TGCGAAC	2525	0.0	16.851484	12
CCCGTAG	2570	0.0	16.772373	19
CCGTAGT	2580	0.0	16.635658	20
GTGCGAA	2600	0.0	16.578848	11
TACGCAT	1010	0.0	16.48515	19
GTAGTCG	2680	0.0	16.152985	22
TCGCGAA	175	1.3278623E-10	15.857142	34
CCCCGTA	2730	0.0	15.789378	18
CGTAGTC	2735	0.0	15.760511	21
ACCCCGT	2830	0.0	15.166078	17
CGAAATG	2865	0.0	15.109948	27
ACGCATT	1145	0.0	14.86463	20
CGGGTTA	1130	0.0	14.570797	34
CTACGCA	1175	0.0	14.485106	18
>>END_MODULE