FastQCFastQC Report
Thu 26 May 2016
SRR1041733_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041733_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8906174
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1244811.3976933304918586No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT1076551.208768209558897No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT796170.8939528915559026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT777360.8728327113303647No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG641410.7201857947082552No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG340640.38247624625344173No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321970.3615132603517515No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG279490.3138160112299625No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC260860.29289793799222874No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT239010.26836439530599787No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG214720.24109118011842123No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTA213720.23996836351950906No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT192780.2164565839382882No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCG177530.19933363080487762No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA174710.19616728799594527No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGC144930.16272980968034084No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT142720.16024838499674496No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT141990.15942872887953907No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG137250.1541065782006954No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG128300.14405736964043145No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCA119750.1344572877197324No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGA119410.13407553007610226No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117280.13168393072041934No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT114150.12816951476582425No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93020.10444440003081008No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG92970.10438825920086449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT5900.014.4237286
CGATGCG3650.014.19178133
CTCGTAT7600.012.9013169
TCTATAC11700.011.85897353
GTACGAC2103.8503822E-711.452383
ACCGTCG5950.011.1932778
TCGTATG8600.011.18604710
TATACCG4700.011.02127555
CGAACGT3700.011.04
ACCGTAT3207.4578566E-1110.9843768
ATTATAC14750.010.7864413
GTATACG1901.8298568E-510.7105261
TATACTG19250.010.6675325
TTAGTAC8050.010.5714293
CGTATCG2104.776606E-610.57142810
AATCTCG9450.010.5714286
TAATACC10100.010.4405944
GAGTACT519150.010.19165911
TAATACT14900.010.1812084
ATAATAC10950.010.1369863
Produced by FastQC (version 0.11.5)