FastQCFastQC Report
Thu 26 May 2016
SRR1041732_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041732_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13685842
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT1964171.4351838929603309No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1463861.0696163232046665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1328100.9704189190551813No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG1027440.7507320338785147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT901070.6583957348038945No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT781160.5707796422025039No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCG714840.5223208042296557No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG579630.42352527524429995No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG433900.31704297039232227No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388350.2837603999812361No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC384870.2812176262154714No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTA357010.26086082244702224No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGC294460.2151566560537525No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT286490.20933311958445813No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG280920.20526322019500154No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257690.18828947462640588No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG213100.1557083590472548No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200890.14678673040358056No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA193280.1412262394962619No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCA189620.13855194294950943No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT188560.13777741990591444No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG183810.13430667985206904No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGA174850.12775976808734166No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT167140.12212620896836306No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT163020.11911579864797503No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG157960.11541854713798391No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG145410.106248486574666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG6750.011.7851855
GTATTAG23700.011.3185651
CGTATAC2952.1100277E-1011.2881363
ATACCGT11200.010.7366086
TAGACTG22300.010.4529145
TATCACG6400.010.406252
CGTTATA2503.239893E-710.362
CGCTTCG10200.010.15686232
TATACTG28250.09.9539825
GAGTACT619550.09.92559111
TATACAG34650.09.8773455
TAATACT24000.09.7895834
AGTACTT631750.09.74855612
GTATAAA34400.09.6802321
GTATAGA21850.09.6521741
GTATAAC19050.09.6141731
TTAATAC22750.09.5956043
ATTATAC27200.09.52205853
TCTATAC18750.09.4723
TATAACG6450.09.4651162
Produced by FastQC (version 0.11.5)