##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041732_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13685842
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.511371898053476	34.0	31.0	34.0	31.0	34.0
2	32.678098578077986	34.0	31.0	34.0	31.0	34.0
3	32.74909720571084	34.0	31.0	34.0	31.0	34.0
4	36.181731018084236	37.0	35.0	37.0	35.0	37.0
5	36.16122851630174	37.0	35.0	37.0	35.0	37.0
6	36.25741923660963	37.0	37.0	37.0	35.0	37.0
7	36.210300104297566	37.0	37.0	37.0	35.0	37.0
8	36.164040838700316	37.0	37.0	37.0	35.0	37.0
9	37.93226145676678	39.0	38.0	39.0	35.0	39.0
10	37.88724486224523	39.0	38.0	39.0	35.0	39.0
11	37.94839820597081	39.0	38.0	39.0	35.0	39.0
12	37.88343201682439	39.0	38.0	39.0	35.0	39.0
13	37.86357178462239	39.0	38.0	39.0	35.0	39.0
14	38.72271468573143	40.0	39.0	40.0	36.0	40.0
15	38.70166132270123	40.0	39.0	40.0	36.0	40.0
16	38.633815734537926	40.0	39.0	40.0	36.0	40.0
17	38.694159044069046	40.0	39.0	40.0	36.0	40.0
18	38.68905910209982	40.0	39.0	40.0	36.0	40.0
19	38.723489647184294	40.0	39.0	40.0	36.0	40.0
20	38.707053099107824	40.0	39.0	40.0	36.0	40.0
21	38.675903316726874	40.0	39.0	40.0	36.0	40.0
22	38.588744411925845	40.0	39.0	40.0	35.0	40.0
23	38.535437790382204	40.0	39.0	40.0	35.0	40.0
24	38.50994019951421	40.0	38.0	40.0	35.0	40.0
25	38.44162405206782	40.0	38.0	40.0	35.0	40.0
26	38.32347107324489	40.0	38.0	40.0	35.0	40.0
27	38.215767798576074	40.0	38.0	40.0	35.0	40.0
28	38.139599814172925	40.0	38.0	40.0	34.0	40.0
29	37.95247460843111	40.0	38.0	40.0	34.0	40.0
30	37.89515668820376	40.0	38.0	40.0	34.0	40.0
31	37.932930615449166	40.0	38.0	40.0	34.0	40.0
32	37.89615136576909	40.0	38.0	40.0	34.0	40.0
33	37.85827587370949	40.0	38.0	40.0	34.0	40.0
34	37.83180947142309	40.0	38.0	40.0	34.0	40.0
35	37.80301635807282	40.0	38.0	40.0	34.0	40.0
36	37.7679363096549	40.0	38.0	40.0	34.0	40.0
37	37.71404616537294	40.0	38.0	40.0	34.0	40.0
38	37.641432730262416	40.0	38.0	40.0	34.0	40.0
39	37.5774761245965	40.0	38.0	40.0	33.0	40.0
40	37.457277455051724	40.0	37.0	40.0	33.0	40.0
41	37.37588027101292	40.0	37.0	40.0	33.0	40.0
42	37.25477270598331	40.0	37.0	40.0	33.0	40.0
43	36.88954957977741	40.0	36.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	8.0
12	10.0
13	29.0
14	30.0
15	45.0
16	57.0
17	163.0
18	260.0
19	453.0
20	925.0
21	1937.0
22	4007.0
23	7748.0
24	13815.0
25	23514.0
26	37961.0
27	59060.0
28	88500.0
29	123063.0
30	160772.0
31	198807.0
32	248262.0
33	315380.0
34	422069.0
35	583633.0
36	903611.0
37	1731255.0
38	5930570.0
39	2829896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.38382804653159	18.680823583963633	14.1970877641288	21.738260605375977
2	15.09833300720555	23.121478386203787	39.05140801713187	22.7287805894588
3	18.732592411924674	27.950205767390855	31.207557415904695	22.10964440477977
4	12.829097398610914	16.881336201309352	36.53637825133448	33.75318814874525
5	14.72413608165285	37.17137754476487	32.89221810393544	15.212268269646836
6	31.755503241963485	36.70767205992879	15.234298335462299	16.302526362645427
7	26.573615273360602	33.20467239063552	22.354795561719914	17.866916774283965
8	26.55635656176653	35.488010164080514	20.457236025375714	17.498397248777238
9	26.534567621049547	16.841455571385378	19.936646937762397	36.687329869802674
10	15.39835839110228	29.26314654224417	31.739245564869158	23.5992495017844
11	32.2807540814807	24.450077678815816	22.801907255688032	20.46726098401545
12	23.41373661920107	26.54366461340121	29.70884071290608	20.33375805449164
13	29.528011502690156	21.8878312346438	27.338208346991	21.245948915675044
14	23.725971701266168	21.554464825766654	29.140304264801536	25.579259208165634
15	25.536667747589075	27.969729593546383	25.68967988962608	20.803922769238458
16	22.884043232414932	26.75652692760884	27.995763797360805	22.363666042615428
17	22.313058999219777	28.21159268096183	28.5432127595803	20.93213556023809
18	22.671590100192592	24.827694196674198	31.754801787131548	20.74591391600166
19	23.672069281524657	27.412957127519082	28.76758331712437	20.147390273831892
20	22.20297443153297	27.52364816136267	29.554615638555525	20.71876176854884
21	21.426595455361827	28.618268426597353	29.00938794997049	20.94574816807033
22	21.398339977912944	27.670719857791724	28.07729330793093	22.85364685636441
23	22.158234765533606	26.071709727468722	28.367176824049263	23.40287868294841
24	20.696388282138578	26.754999801985146	28.656738840036294	23.89187307583998
25	22.302142608397787	27.304903856116415	28.505027312166835	21.88792622331896
26	22.646900351472713	25.650873362413506	29.608423069621875	22.093803216491906
27	22.53618009034446	27.550456888220687	29.99019716872371	19.923165852711143
28	24.394670053914112	25.996383708068528	29.151206041981194	20.457740196036166
29	21.36822126106673	26.5455790005467	31.00356558259258	21.082634155793993
30	21.459074275444653	27.419832846236282	30.56908007559929	20.552012802719773
31	21.344817512872062	25.547160342783442	30.509829062764275	22.59819308158022
32	21.48369826277404	26.624945692051682	29.27010994281536	22.62124610235892
33	22.851206378094968	26.697853153645934	28.205586473963386	22.245353994295712
34	22.52717808666796	27.870473734827566	28.392312288860268	21.210035889644203
35	21.839569680842434	28.088735789876868	29.400090984537158	20.67160354474354
36	22.734713728245584	27.56789096352274	28.093507144098258	21.603888164133416
37	20.545064015790917	26.914237355655573	29.086336083669533	23.454362544883974
38	22.005836396474546	27.857759865998744	28.64051039022663	21.49589334730008
39	22.204618466295315	26.69468930008106	28.166823787677806	22.93386844594582
40	22.32719769817597	26.8329124360781	30.501053570543924	20.338836295202007
41	24.316852408496313	25.873037259965443	28.327990342136054	21.482119989402186
42	22.93171293370185	26.062064723529616	29.783392209262683	21.222830133505852
43	23.26665761595085	25.625979022700978	29.975422776326077	21.1319405850221
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18773.0
1	14545.0
2	10317.0
3	24786.0
4	39255.0
5	39255.0
6	41347.5
7	43440.0
8	36299.0
9	29158.0
10	37663.0
11	46168.0
12	46168.0
13	71119.5
14	96071.0
15	172664.5
16	249258.0
17	240878.0
18	232498.0
19	232498.0
20	220420.5
21	208343.0
22	183953.5
23	159564.0
24	185962.0
25	212360.0
26	212360.0
27	239483.5
28	266607.0
29	305762.0
30	344917.0
31	388080.0
32	431243.0
33	431243.0
34	476335.5
35	521428.0
36	581542.0
37	641656.0
38	692615.5
39	743575.0
40	743575.0
41	786930.0
42	830285.0
43	867868.0
44	905451.0
45	945344.0
46	985237.0
47	985237.0
48	1083159.0
49	1181081.0
50	1209193.5
51	1237306.0
52	1365570.5
53	1493835.0
54	1493835.0
55	1234789.0
56	975743.0
57	762675.5
58	549608.0
59	484479.0
60	419350.0
61	419350.0
62	356500.5
63	293651.0
64	242276.0
65	190901.0
66	159113.5
67	127326.0
68	127326.0
69	101567.5
70	75809.0
71	63760.5
72	51712.0
73	40451.5
74	29191.0
75	29191.0
76	23033.5
77	16876.0
78	13340.5
79	9805.0
80	8693.5
81	7582.0
82	7582.0
83	6486.5
84	5391.0
85	4312.5
86	3234.0
87	2184.5
88	1135.0
89	1135.0
90	769.5
91	404.0
92	272.0
93	140.0
94	136.0
95	132.0
96	132.0
97	73.5
98	15.0
99	13.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.3685842E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.474579589519195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.87834394809792	18.85716517513954
2	17.953774416934152	10.94267454802267
3	7.529670228977461	6.883906040274207
4	3.664864633396006	4.467408358209627
5	2.118962907418192	3.228725188467734
6	1.338521029930336	2.447451939531434
7	0.933488084843433	1.9913359856200334
8	0.6778445974438874	1.6525623307303476
9	0.4922973843501233	1.350230023898096
>10	2.9751028180288235	16.937002710066334
>50	0.26689228201476944	5.610774853236837
>100	0.15311531839267695	8.64496045656651
>500	0.010051094390696569	2.050639904023627
>1k	0.005595110591376483	3.107296169949164
>5k	6.428374213540547E-4	1.4158418106133897
>10k+	8.333077684219227E-4	10.412024505650393
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT	196417	1.4351838929603309	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	146386	1.0696163232046665	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	132810	0.9704189190551813	No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG	102744	0.7507320338785147	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	90107	0.6583957348038945	No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT	78116	0.5707796422025039	No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCG	71484	0.5223208042296557	No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG	57963	0.42352527524429995	No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG	43390	0.31704297039232227	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38835	0.2837603999812361	No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC	38487	0.2812176262154714	No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTA	35701	0.26086082244702224	No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGC	29446	0.2151566560537525	No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT	28649	0.20933311958445813	No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG	28092	0.20526322019500154	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25769	0.18828947462640588	No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG	21310	0.1557083590472548	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20089	0.14678673040358056	No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA	19328	0.1412262394962619	No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCA	18962	0.13855194294950943	No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT	18856	0.13777741990591444	No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG	18381	0.13430667985206904	No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGA	17485	0.12775976808734166	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16714	0.12212620896836306	No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT	16302	0.11911579864797503	No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG	15796	0.11541854713798391	No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG	14541	0.106248486574666	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4613642331980744E-5	0.0	0.0	0.0	7.306821165990372E-6
2	4.384092699594223E-5	0.0	0.0	1.4613642331980744E-5	7.306821165990372E-6
3	5.1147748161932604E-5	0.0	0.0	2.1920463497971114E-5	7.306821165990372E-6
4	5.1147748161932604E-5	0.0	0.0	5.8454569327922974E-5	1.4613642331980744E-5
5	1.6805688681777856E-4	0.0	0.0	8.037503282589409E-5	1.4613642331980744E-5
6	2.0459099264773042E-4	0.0	0.0	1.0229549632386521E-4	1.4613642331980744E-5
7	2.1189781381372077E-4	0.0	0.0	1.315227809878267E-4	1.4613642331980744E-5
8	2.776592043076341E-4	0.0	0.0	1.315227809878267E-4	1.4613642331980744E-5
9	3.507274159675378E-4	0.0	0.0	1.8997735031574966E-4	2.1920463497971114E-5
10	4.7494337578937414E-4	0.0	0.0	2.2651145614570152E-4	2.1920463497971114E-5
11	4.968638392873453E-4	0.0	0.0	3.068864889715956E-4	2.1920463497971114E-5
12	5.041706604533356E-4	0.0	0.0	4.0918198529546083E-4	2.1920463497971114E-5
13	5.553184086152682E-4	0.0	0.0	4.164888064614512E-4	2.1920463497971114E-5
14	6.576139049391334E-4	7.306821165990372E-6	0.0	4.676365546233838E-4	2.9227284663961487E-5
15	6.868411896030949E-4	7.306821165990372E-6	0.0	5.187843027853163E-4	5.1147748161932604E-5
16	7.233752954330468E-4	7.306821165990372E-6	0.0	5.991593356112105E-4	5.1147748161932604E-5
17	7.45295758931018E-4	7.306821165990372E-6	0.0	7.014548319350756E-4	5.1147748161932604E-5
18	7.45295758931018E-4	7.306821165990372E-6	0.0	8.402844340888927E-4	5.8454569327922974E-5
19	7.891366859269602E-4	7.306821165990372E-6	0.0	0.0010887163537325654	5.8454569327922974E-5
20	9.498867515787483E-4	7.306821165990372E-6	0.0	0.001512511981360007	5.8454569327922974E-5
21	0.0013809892003721802	7.306821165990372E-6	0.0	0.0021920463497971114	5.8454569327922974E-5
22	0.0014029096638701513	7.306821165990372E-6	0.0	0.0034707400538454268	5.8454569327922974E-5
23	0.0014175233062021322	7.306821165990372E-6	0.0	0.005662786403642538	8.037503282589409E-5
24	0.0014394437697001032	7.306821165990372E-6	0.0	0.010119947314896665	8.037503282589409E-5
25	0.001454057412032084	7.306821165990372E-6	1.4613642331980744E-5	0.014620949153146734	8.037503282589409E-5
26	0.0016586484046798144	7.306821165990372E-6	1.4613642331980744E-5	0.020356803768449176	8.768185399188445E-5
27	0.0016951825105097661	7.306821165990372E-6	1.4613642331980744E-5	0.026509147190213068	8.768185399188445E-5
28	0.0017536370798376891	7.306821165990372E-6	1.4613642331980744E-5	0.04307371077351324	8.768185399188445E-5
29	0.0017609439010036795	7.306821165990372E-6	1.4613642331980744E-5	0.08188754480725409	8.768185399188445E-5
30	0.001790171185667641	7.306821165990372E-6	1.4613642331980744E-5	0.1345697254140447	8.768185399188445E-5
31	0.0018559325761615545	7.306821165990372E-6	1.4613642331980744E-5	0.18158181279602673	1.0229549632386521E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	675	0.0	11.785185	5
GTATTAG	2370	0.0	11.318565	1
CGTATAC	295	2.1100277E-10	11.288136	3
ATACCGT	1120	0.0	10.736608	6
TAGACTG	2230	0.0	10.452914	5
TATCACG	640	0.0	10.40625	2
CGTTATA	250	3.239893E-7	10.36	2
CGCTTCG	1020	0.0	10.156862	32
TATACTG	2825	0.0	9.953982	5
GAGTACT	61955	0.0	9.925591	11
TATACAG	3465	0.0	9.877345	5
TAATACT	2400	0.0	9.789583	4
AGTACTT	63175	0.0	9.748556	12
GTATAAA	3440	0.0	9.680232	1
GTATAGA	2185	0.0	9.652174	1
GTATAAC	1905	0.0	9.614173	1
TTAATAC	2275	0.0	9.595604	3
ATTATAC	2720	0.0	9.5220585	3
TCTATAC	1875	0.0	9.472	3
TATAACG	645	0.0	9.465116	2
>>END_MODULE