FastQCFastQC Report
Thu 26 May 2016
SRR1041731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14232968
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1001310.7035145445419395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT893800.6279786478828591No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT653190.4589274703631737No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220110.15464799752237202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA232350.022.508931
GTATCAA458150.018.0295751
TATCAAC664150.012.3537622
AACGCAG683300.011.9506816
ATCAACG687850.011.8259083
TCAACGC696600.011.68267254
CAACGCA708300.011.5314845
ACGCAGA723200.011.2836697
CGCAGAG737150.011.0701358
AGAGTAC750750.010.82277711
CAGAGTA764800.010.66749510
GCAGAGT776850.010.5210789
TACGGAT24100.010.28630727
TAATACC17450.010.2836684
TATACCG6300.010.2777785
CGTTACG12450.010.25301224
GTATTAA24800.010.0705641
TCGTTAC16850.09.99109723
TAGATCG4650.09.9462385
GTATAAA31100.09.9340841
Produced by FastQC (version 0.11.5)