##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041731_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14232968
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53389820029104	34.0	31.0	34.0	31.0	34.0
2	32.73095428866277	34.0	31.0	34.0	31.0	34.0
3	32.793294413364805	34.0	31.0	34.0	31.0	34.0
4	36.23199230125439	37.0	37.0	37.0	35.0	37.0
5	36.20169854945223	37.0	37.0	37.0	35.0	37.0
6	36.26069678509781	37.0	37.0	37.0	35.0	37.0
7	36.226509186277944	37.0	37.0	37.0	35.0	37.0
8	36.17530278997325	37.0	37.0	37.0	35.0	37.0
9	37.948741752247315	39.0	38.0	39.0	35.0	39.0
10	37.878858155235086	39.0	38.0	39.0	35.0	39.0
11	37.95148798198661	39.0	38.0	39.0	35.0	39.0
12	37.86613185668653	39.0	38.0	39.0	35.0	39.0
13	37.87970534325659	39.0	38.0	39.0	35.0	39.0
14	38.77267018375928	40.0	39.0	40.0	36.0	40.0
15	38.75438460902884	40.0	39.0	40.0	36.0	40.0
16	38.74098115024217	40.0	39.0	40.0	36.0	40.0
17	38.736971375190336	40.0	39.0	40.0	36.0	40.0
18	38.71539632492675	40.0	39.0	40.0	36.0	40.0
19	38.71612828750827	40.0	39.0	40.0	36.0	40.0
20	38.68086333082461	40.0	39.0	40.0	36.0	40.0
21	38.64870384026719	40.0	39.0	40.0	36.0	40.0
22	38.559650594310334	40.0	39.0	40.0	35.0	40.0
23	38.522830937299936	40.0	39.0	40.0	35.0	40.0
24	38.49010297781882	40.0	38.0	40.0	35.0	40.0
25	38.42707374877819	40.0	38.0	40.0	35.0	40.0
26	38.31764204064816	40.0	38.0	40.0	35.0	40.0
27	38.23685952220226	40.0	38.0	40.0	34.0	40.0
28	38.16074988716338	40.0	38.0	40.0	34.0	40.0
29	38.090041304104666	40.0	38.0	40.0	34.0	40.0
30	38.005433371310886	40.0	38.0	40.0	34.0	40.0
31	38.07703467049178	40.0	38.0	40.0	34.0	40.0
32	38.048362857276146	40.0	38.0	40.0	34.0	40.0
33	38.01272264505899	40.0	38.0	40.0	34.0	40.0
34	38.03080615371299	40.0	38.0	40.0	34.0	40.0
35	38.03819610920224	40.0	38.0	40.0	34.0	40.0
36	38.02514949798243	40.0	38.0	40.0	34.0	40.0
37	37.9884263071483	40.0	38.0	40.0	34.0	40.0
38	37.934649540419116	40.0	38.0	40.0	34.0	40.0
39	37.873691980478	40.0	38.0	40.0	34.0	40.0
40	37.8277008000018	40.0	38.0	40.0	34.0	40.0
41	37.75358365170216	40.0	38.0	40.0	33.0	40.0
42	37.685683899521166	40.0	38.0	40.0	33.0	40.0
43	37.4150950104012	40.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	9.0
13	8.0
14	20.0
15	23.0
16	61.0
17	108.0
18	204.0
19	411.0
20	880.0
21	1885.0
22	4002.0
23	7622.0
24	13660.0
25	22411.0
26	35866.0
27	53628.0
28	77800.0
29	108980.0
30	145532.0
31	190576.0
32	244268.0
33	315957.0
34	424519.0
35	591288.0
36	906387.0
37	1677712.0
38	6264196.0
39	3144951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.31315155068149	18.646855666365582	14.001366405095549	24.03862637785738
2	17.019682753449597	21.346250479871802	37.2967816691501	24.3372850975285
3	18.41429700396994	24.615399964364425	30.74696718210847	26.223335849557166
4	13.827867806630353	17.159379547540613	35.79476887744004	33.217983768389
5	14.395556850826896	37.09850257514806	33.74329935962759	14.762641214397446
6	32.81145576945019	36.604881005845016	15.869437772922696	14.714225451782086
7	28.399712554682903	32.087383320190135	21.121329015845465	18.391575109281494
8	27.285222590256648	34.4987496634574	19.11866168742879	19.097366058857155
9	27.045911998115923	14.968388884173702	18.994562483383646	38.99113663432673
10	15.77631594478397	28.76347364794188	32.090615253262705	23.369595154011446
11	34.994774104740486	22.734822420734734	22.628295096286312	19.64210837823847
12	23.455480262444205	25.169353293002555	29.46473989121594	21.910426553337295
13	29.517476607830496	21.599915070419605	25.345395282277032	23.537213039472864
14	22.693151561922996	20.77350275782254	27.130265451309942	29.403080228944518
15	24.177016346836446	28.538439768852147	23.937958688588353	23.34658519572306
16	23.620435316091484	27.473995585460457	25.089903946949082	23.815665151498973
17	23.279304780281947	26.924967441787263	26.29840100813829	23.497326769792497
18	24.03772705735023	25.93897492076143	27.227616896208858	22.79568112567948
19	25.352779546753705	25.705755819868354	27.226000929672573	21.71546370370537
20	24.526936335415073	25.482780541627015	27.40082743107411	22.5894556918838
21	24.04209719293966	25.64728593502072	27.653269507807508	22.657347364232113
22	23.876720582804655	25.29929808034417	27.445554574421866	23.37842676242931
23	23.299785399643984	25.313638026868325	27.786186268387592	23.600390305100102
24	23.127825482358986	25.53746344402657	27.73007007392977	23.604640999684676
25	23.595099771179136	25.36614991335609	27.692172145683173	23.346578169781594
26	23.414371478949437	25.706008753760983	27.751133846433156	23.128485920856424
27	23.577232802041006	25.909627563274224	27.434594105740985	23.078545528943785
28	23.487005661784668	25.816273879067246	27.464229526828138	23.232490932319948
29	23.506298897039603	26.045129870312362	27.567271984311354	22.881299248336678
30	23.89480535612811	25.830058776215896	27.300194871512396	22.9749409961436
31	23.78441376387553	25.570288642537527	27.592150843028662	23.05314675055828
32	23.549396021968153	25.937197357571517	27.277205991048387	23.23620062941194
33	23.43035549577572	25.55912442155424	27.421877151694574	23.588642930975464
34	23.643508507853035	25.772846534889982	27.527350584923678	23.056294372333305
35	23.551145481392215	25.801041637977406	27.367320716241334	23.280492164389045
36	23.57530066813893	25.839571900955583	27.41639691735413	23.168730513551356
37	23.49779750787046	25.777701460440294	27.491230219867003	23.233270811822244
38	23.528732728128105	25.777497708137897	27.37868868952702	23.31508087420698
39	23.330179622409045	26.03864492634284	27.409581754135893	23.221593697112226
40	23.80875865104172	25.590741158133707	27.443095494910125	23.157404695914444
41	23.460117383809195	25.754958487927464	27.502928412401406	23.28199571586193
42	23.54768871819286	25.709606035789584	27.412462390135357	23.3302428558822
43	23.46483881647173	25.457255296295195	27.413523307296135	23.664382579936944
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15159.0
1	11349.5
2	7540.0
3	11289.5
4	15039.0
5	15039.0
6	16977.5
7	18916.0
8	15918.5
9	12921.0
10	17782.5
11	22644.0
12	22644.0
13	36765.5
14	50887.0
15	97110.0
16	143333.0
17	148282.5
18	153232.0
19	153232.0
20	146351.5
21	139471.0
22	127196.0
23	114921.0
24	138475.0
25	162029.0
26	162029.0
27	190879.0
28	219729.0
29	258181.5
30	296634.0
31	343052.0
32	389470.0
33	389470.0
34	442367.0
35	495264.0
36	561413.0
37	627562.0
38	693705.5
39	759849.0
40	759849.0
41	824098.0
42	888347.0
43	953034.0
44	1017721.0
45	1072371.5
46	1127022.0
47	1127022.0
48	1169995.5
49	1212969.0
50	1211950.0
51	1210931.0
52	1177616.5
53	1144302.0
54	1144302.0
55	1077068.5
56	1009835.0
57	925072.5
58	840310.0
59	765174.0
60	690038.0
61	690038.0
62	598678.0
63	507318.0
64	423031.5
65	338745.0
66	283013.5
67	227282.0
68	227282.0
69	183909.5
70	140537.0
71	118038.0
72	95539.0
73	76589.0
74	57639.0
75	57639.0
76	45576.0
77	33513.0
78	26693.0
79	19873.0
80	16216.0
81	12559.0
82	12559.0
83	9929.0
84	7299.0
85	5738.5
86	4178.0
87	2871.0
88	1564.0
89	1564.0
90	1046.0
91	528.0
92	357.0
93	186.0
94	147.5
95	109.0
96	109.0
97	60.5
98	12.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.4232968E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.53357164878241
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.69170902261864	25.698697480028237
2	15.941364861752755	12.285554501594644
3	6.108431813690575	7.061390848636432
4	3.016577541289225	4.64958027285505
5	1.8248414483927862	3.515882934965565
6	1.221971454735837	2.825215476229815
7	0.8432462470031574	2.274530277352413
8	0.6444902064259197	1.9867607637001399
9	0.4873780258622981	1.6902374471646293
>10	2.8703315689314817	20.692029321515975
>50	0.23504148110909528	6.216357997455293
>100	0.11006539226763046	7.339863397839731
>500	0.00341949445289262	0.8869828866828235
>1k	9.489626532832608E-4	0.6007229227306742
>5k	9.12394072098694E-5	0.2556605071224717
>10k+	9.12394072098694E-5	2.0205329641259797
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	100131	0.7035145445419395	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	89380	0.6279786478828591	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	65319	0.4589274703631737	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22011	0.15464799752237202	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.025941462104039E-6	2.1077824386312116E-5
2	0.0	0.0	0.0	3.5129707310520194E-5	2.1077824386312116E-5
3	0.0	0.0	0.0	4.215564877262423E-5	2.1077824386312116E-5
4	0.0	0.0	0.0	6.323347315893636E-5	3.5129707310520194E-5
5	7.025941462104039E-6	2.1077824386312116E-5	0.0	9.133723900735251E-5	3.5129707310520194E-5
6	7.728535608314443E-5	2.1077824386312116E-5	0.0	9.836318046945655E-5	6.323347315893636E-5
7	7.728535608314443E-5	2.1077824386312116E-5	0.0	1.1241506339366463E-4	6.323347315893636E-5
8	1.0538912193156058E-4	2.1077824386312116E-5	0.0	1.4754477070418482E-4	7.025941462104039E-5
9	1.1241506339366463E-4	2.1077824386312116E-5	0.0	2.599598340978494E-4	8.431129754524846E-5
10	1.615966536283929E-4	2.1077824386312116E-5	0.0	3.58323014567306E-4	8.431129754524846E-5
11	2.0375230240101712E-4	2.1077824386312116E-5	0.0	4.4263431211255446E-4	8.431129754524846E-5
12	2.1077824386312116E-4	2.8103765848416157E-5	0.0	5.128937267335948E-4	9.836318046945655E-5
13	2.178041853252252E-4	3.5129707310520194E-5	0.0	5.40997492582011E-4	1.1944100485576866E-4
14	2.318560682494333E-4	3.5129707310520194E-5	0.0	5.620753169683231E-4	1.3349288777997673E-4
15	2.3888200971153731E-4	3.5129707310520194E-5	0.0	5.761271998925312E-4	1.6862259509049693E-4
16	2.599598340978494E-4	3.5129707310520194E-5	0.0	5.972050242788433E-4	1.7564853655260097E-4
17	2.6698577555995346E-4	3.5129707310520194E-5	0.0	6.393606730514676E-4	1.8970041947680906E-4
18	2.740117170220575E-4	3.5129707310520194E-5	0.0	6.744903803619877E-4	2.0375230240101712E-4
19	2.740117170220575E-4	3.5129707310520194E-5	0.0	7.447497949830281E-4	2.0375230240101712E-4
20	3.231933072567858E-4	3.5129707310520194E-5	0.0	8.782426827630049E-4	2.0375230240101712E-4
21	4.0047866333993025E-4	3.5129707310520194E-5	0.0	0.0013279029363376634	2.0375230240101712E-4
22	4.145305462641383E-4	3.5129707310520194E-5	0.0	0.0021007564971691076	2.1077824386312116E-4
23	4.145305462641383E-4	3.5129707310520194E-5	0.0	0.0026628318141374307	2.2483012678732925E-4
24	4.2155648772624233E-4	4.9181590234728276E-5	0.0	0.0038361640383088053	2.2483012678732925E-4
25	4.2858242918834637E-4	4.9181590234728276E-5	0.0	0.004299876174807672	2.318560682494333E-4
26	4.7073807796097064E-4	4.9181590234728276E-5	0.0	0.005571571579448503	2.318560682494333E-4
27	4.7073807796097064E-4	5.620753169683231E-5	0.0	0.008374922222828015	2.318560682494333E-4
28	4.7776401942307463E-4	5.620753169683231E-5	0.0	0.019785051157284975	2.318560682494333E-4
29	4.8478996088517867E-4	5.620753169683231E-5	0.0	0.04307604710415986	2.318560682494333E-4
30	4.918159023472828E-4	5.620753169683231E-5	0.0	0.0720158999865664	2.318560682494333E-4
31	4.988418438093868E-4	5.620753169683231E-5	0.0	0.11197945502301418	2.3888200971153731E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	23235	0.0	22.50893	1
GTATCAA	45815	0.0	18.029575	1
TATCAAC	66415	0.0	12.353762	2
AACGCAG	68330	0.0	11.950681	6
ATCAACG	68785	0.0	11.825908	3
TCAACGC	69660	0.0	11.6826725	4
CAACGCA	70830	0.0	11.531484	5
ACGCAGA	72320	0.0	11.283669	7
CGCAGAG	73715	0.0	11.070135	8
AGAGTAC	75075	0.0	10.822777	11
CAGAGTA	76480	0.0	10.667495	10
GCAGAGT	77685	0.0	10.521078	9
TACGGAT	2410	0.0	10.286307	27
TAATACC	1745	0.0	10.283668	4
TATACCG	630	0.0	10.277778	5
CGTTACG	1245	0.0	10.253012	24
GTATTAA	2480	0.0	10.070564	1
TCGTTAC	1685	0.0	9.991097	23
TAGATCG	465	0.0	9.946238	5
GTATAAA	3110	0.0	9.934084	1
>>END_MODULE