FastQCFastQC Report
Thu 26 May 2016
SRR1041730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12679743
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT921010.726363302473875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT880710.6945803239071959No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT606410.47825101817915394No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207110.16333927272816176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA239250.020.8158841
GTATCAA449750.016.9225141
TATCAAC656600.011.5801091
AACGCAG666100.011.5149385
ATCAACG677000.011.2666912
TCAACGC685650.011.1704233
CAACGCA692250.011.0692674
ACGCAGA709450.010.8504476
TTATACT25150.010.8131224
CGCAGAG720550.010.6832987
AGAGTAC732400.010.53317910
GTATTAT17950.010.5125351
CAGAGTA751100.010.3817739
GCAGAGT759500.010.2109288
TACGGAT20400.010.15686227
CGTTATA2952.8559953E-810.0338982
TATACAG31950.010.0172145
ATTATAC19600.010.0051023
TATACTG29150.09.963985
GTATTAC13100.09.8854961
Produced by FastQC (version 0.11.5)