##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041730_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12679743
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34516780032529	33.0	31.0	34.0	31.0	34.0
2	32.56651905326472	34.0	31.0	34.0	31.0	34.0
3	32.63086901682471	34.0	31.0	34.0	31.0	34.0
4	36.13883633130419	37.0	35.0	37.0	35.0	37.0
5	36.098244262521725	37.0	35.0	37.0	35.0	37.0
6	36.1695763076586	37.0	36.0	37.0	35.0	37.0
7	36.1335504197522	37.0	36.0	37.0	35.0	37.0
8	36.08260687933502	37.0	36.0	37.0	35.0	37.0
9	37.826953827061004	39.0	38.0	39.0	35.0	39.0
10	37.73175883769884	39.0	38.0	39.0	35.0	39.0
11	37.81561471711217	39.0	38.0	39.0	35.0	39.0
12	37.72059307511201	39.0	38.0	39.0	35.0	39.0
13	37.735864756880325	39.0	38.0	39.0	35.0	39.0
14	38.618475469100595	40.0	38.0	40.0	36.0	40.0
15	38.60103536798814	40.0	38.0	40.0	36.0	40.0
16	38.58490523033472	40.0	38.0	40.0	36.0	40.0
17	38.5807092462363	40.0	38.0	40.0	36.0	40.0
18	38.558108472703275	40.0	38.0	40.0	36.0	40.0
19	38.56170744154672	40.0	39.0	40.0	35.0	40.0
20	38.51994768348223	40.0	38.0	40.0	35.0	40.0
21	38.48447504022755	40.0	38.0	40.0	35.0	40.0
22	38.39160659644285	40.0	38.0	40.0	35.0	40.0
23	38.35182858201463	40.0	38.0	40.0	35.0	40.0
24	38.314502431161266	40.0	38.0	40.0	35.0	40.0
25	38.24449257370595	40.0	38.0	40.0	34.0	40.0
26	38.125268548424046	40.0	38.0	40.0	34.0	40.0
27	38.03789185632548	40.0	38.0	40.0	34.0	40.0
28	37.95578703763949	40.0	38.0	40.0	34.0	40.0
29	37.87649489425772	40.0	38.0	40.0	34.0	40.0
30	37.78829097719094	40.0	38.0	40.0	34.0	40.0
31	37.871201727038155	40.0	38.0	40.0	34.0	40.0
32	37.84302773329081	40.0	38.0	40.0	34.0	40.0
33	37.80438286485775	40.0	38.0	40.0	34.0	40.0
34	37.838869368251395	40.0	38.0	40.0	34.0	40.0
35	37.85190086265944	40.0	38.0	40.0	34.0	40.0
36	37.83770436041172	40.0	38.0	40.0	34.0	40.0
37	37.79603703324271	40.0	38.0	40.0	34.0	40.0
38	37.74202592276515	40.0	38.0	40.0	33.0	40.0
39	37.6793915302542	40.0	38.0	40.0	33.0	40.0
40	37.63223276686286	40.0	38.0	40.0	33.0	40.0
41	37.55279164569818	40.0	37.0	40.0	33.0	40.0
42	37.48254235121327	40.0	37.0	40.0	33.0	40.0
43	37.180188510129895	40.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	9.0
13	15.0
14	19.0
15	24.0
16	70.0
17	116.0
18	233.0
19	407.0
20	896.0
21	1997.0
22	4058.0
23	7750.0
24	13795.0
25	22997.0
26	36200.0
27	53930.0
28	78396.0
29	108441.0
30	144468.0
31	187658.0
32	241938.0
33	310356.0
34	417397.0
35	578850.0
36	892747.0
37	1675715.0
38	5899468.0
39	2001790.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.38656098944592	19.591919173756125	14.559348718660939	23.462171118137015
2	16.97339606962065	22.052197745648314	37.348477804321426	23.625928380409604
3	18.33302141849405	25.526014210224922	31.148833221619714	24.992131149661315
4	13.87432694810928	17.779445529771383	36.246113190149046	32.10011433197029
5	14.034480036385597	37.770300234003166	33.90235906200938	14.29286066760186
6	32.188160280535655	37.63148827227808	15.810438744696956	14.369912702489318
7	27.86531241208911	32.53584871554573	21.525444167125467	18.073394705239686
8	27.01113894816322	35.03756345850228	19.254546405238653	18.69675118809585
9	26.847752355863996	15.085069153215489	19.231525433914552	38.83565305700596
10	15.42640887910741	29.184771331721787	32.43592555464255	22.952894234528255
11	34.71668156050166	23.02745410533952	22.92539367714314	19.330470657015685
12	23.175067507283075	25.58119671668424	29.787464935212014	21.45627084082067
13	29.26601903524385	21.874047447176174	25.763724075480077	23.09620944209989
14	22.44931147263789	21.045363458865058	27.594463073896687	28.910861994600364
15	23.95004378243313	29.10829501828231	24.07330337846753	22.86835782081703
16	23.291063549158686	27.82763026032941	25.34922829271855	23.532077897793354
17	22.965954436142752	27.31361353301877	26.648647374004348	23.071784656834133
18	23.727247468659264	26.16967078906883	27.659275113068144	22.443806629203763
19	24.991657953950643	26.080433964631617	27.636049090269417	21.29185899114832
20	24.159235719525228	25.8404685331556	27.76917481687129	22.231120930447883
21	23.700488251220865	25.979075443406067	28.009463598749594	22.31097270662347
22	23.431626335013256	25.648461486956002	27.846991851490998	23.07292032653974
23	22.969116960809064	25.59021109497251	28.13180046314819	23.30887148107024
24	22.81479206636917	25.93249721228577	28.04563941083033	23.20707131051473
25	23.300566896347977	25.73476449798707	28.07194909234359	22.892719513321367
26	23.14621045552737	25.97849183536291	28.106452946246623	22.768844762863097
27	23.21729233786521	26.263844622087372	27.833048351216583	22.68581468883084
28	23.26000613734837	26.049439645582723	27.886827043734247	22.803727173334664
29	23.13284267670094	26.365818297736794	27.968579489347693	22.532759536214574
30	23.531667794844108	26.20240015905685	27.676286498866737	22.58964554723231
31	23.48836250072261	25.85246404442109	27.98885592554991	22.67031752930639
32	23.132527212893823	26.309776152403092	27.617271107150987	22.940425527552097
33	23.120760412888494	25.965202922488256	27.746193278523073	23.167843386100177
34	23.34625394221318	26.1004028236219	27.861148289835214	22.692194944329707
35	23.258728508929558	26.18582253599304	27.71161844526344	22.843830509813962
36	23.320622507885215	26.219190720190465	27.65895964926103	22.80122712266329
37	23.16626606706461	26.067428969183364	27.861826537020505	22.90447842673152
38	23.32052786874308	26.076427574281276	27.690853040160203	22.912191516815444
39	23.134191284476348	26.37155185243108	27.613856211438986	22.880400651653588
40	23.48909595407415	25.98647306968288	27.81216464718567	22.7122663290573
41	23.25132299605757	26.024486458440048	27.80505882493044	22.91913172057194
42	23.348485848648508	26.06694000028234	27.674054592431407	22.910519558637745
43	23.203317291210084	25.789836592113897	27.778473112585957	23.228373004090066
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14223.0
1	10699.5
2	7176.0
3	10208.5
4	13241.0
5	13241.0
6	15371.5
7	17502.0
8	14537.0
9	11572.0
10	16124.0
11	20676.0
12	20676.0
13	33606.0
14	46536.0
15	92590.0
16	138644.0
17	139987.5
18	141331.0
19	141331.0
20	135687.0
21	130043.0
22	120595.0
23	111147.0
24	133221.5
25	155296.0
26	155296.0
27	183554.5
28	211813.0
29	249030.0
30	286247.0
31	330586.0
32	374925.0
33	374925.0
34	427430.5
35	479936.0
36	543020.0
37	606104.0
38	667032.5
39	727961.0
40	727961.0
41	781567.0
42	835173.0
43	889068.5
44	942964.0
45	981973.5
46	1020983.0
47	1020983.0
48	1051830.5
49	1082678.0
50	1075886.5
51	1069095.0
52	1033823.5
53	998552.0
54	998552.0
55	927451.5
56	856351.0
57	779143.0
58	701935.0
59	634031.0
60	566127.0
61	566127.0
62	486246.5
63	406366.0
64	334733.5
65	263101.0
66	217130.5
67	171160.0
68	171160.0
69	138345.0
70	105530.0
71	86887.5
72	68245.0
73	54660.5
74	41076.0
75	41076.0
76	31842.0
77	22608.0
78	17805.0
79	13002.0
80	11196.5
81	9391.0
82	9391.0
83	7634.0
84	5877.0
85	4529.5
86	3182.0
87	2227.5
88	1273.0
89	1273.0
90	843.5
91	414.0
92	283.0
93	152.0
94	128.5
95	105.0
96	105.0
97	59.0
98	13.0
99	15.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1.2679743E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.3934362647581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.92461854680653	22.97905348550129
2	16.119249309088612	11.410312461139476
3	6.457932792504799	6.857052980808296
4	3.3678088857233677	4.76793316594944
5	2.001481441735724	3.5419652921584754
6	1.3398974443168894	2.8454144878045255
7	0.9279968623260643	2.2991498460443136
8	0.6826853500509092	1.9330064340728745
9	0.5320095599253016	1.6946681806312351
>10	3.2228218629030088	21.443704254669978
>50	0.2753019641049393	6.720085071829747
>100	0.1408496861135701	8.799064903342668
>500	0.005358389154358873	1.2630359821372137
>1k	0.0017645493745712922	0.998552860712198
>5k	1.1167793564866222E-4	0.2976515527499097
>10k+	1.1167793564866222E-4	2.1493490404483677
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	92101	0.726363302473875	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	88071	0.6945803239071959	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	60641	0.47825101817915394	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20711	0.16333927272816176	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.5773190355672034E-5	0.0
4	0.0	0.0	0.0	3.9432975889180085E-5	0.0
5	3.9432975889180085E-5	0.0	0.0	9.463914213403222E-5	0.0
6	1.1041233248970425E-4	0.0	0.0	1.1041233248970425E-4	1.5773190355672034E-5
7	1.1829892766754026E-4	0.0	0.0	1.4195871320104832E-4	1.5773190355672034E-5
8	1.5773190355672034E-4	0.0	0.0	1.735050939123924E-4	3.154638071134407E-5
9	1.5773190355672034E-4	0.0	0.0	2.5237104569075255E-4	7.886595177836017E-5
10	1.9716487944590043E-4	0.0	0.0	3.627833781804568E-4	7.886595177836017E-5
11	2.5237104569075255E-4	0.0	0.0	4.101029492474729E-4	7.886595177836017E-5
12	2.6025764086858857E-4	0.0	0.0	4.7319571067016105E-4	7.886595177836017E-5
13	2.8391742640209664E-4	0.0	0.0	4.810823058479971E-4	7.886595177836017E-5
14	3.075772119356047E-4	0.0	0.0	4.968554962036691E-4	1.1041233248970425E-4
15	3.233504022912767E-4	0.0	0.0	5.047420913815051E-4	1.4195871320104832E-4
16	3.627833781804568E-4	0.0	0.0	5.205152817371771E-4	1.5773190355672034E-4
17	3.7066997335829285E-4	0.0	0.0	5.757214479820293E-4	1.5773190355672034E-4
18	3.7066997335829285E-4	0.0	0.0	6.309276142268814E-4	1.813916890902284E-4
19	3.785565685361289E-4	0.0	0.0	6.467008045825534E-4	1.9716487944590043E-4
20	4.65309115492325E-4	0.0	0.0	7.492265418944217E-4	2.0505147462373646E-4
21	5.914946383377013E-4	0.0	0.0	0.0010725769441856984	2.208246649794085E-4
22	5.914946383377013E-4	0.0	0.0	0.0015221128693223515	2.208246649794085E-4
23	5.993812335155374E-4	0.0	0.0	0.0019716487944590045	3.233504022912767E-4
24	6.230410190490454E-4	0.0	0.0	0.003067885524178211	3.233504022912767E-4
25	6.230410190490454E-4	0.0	0.0	0.0035174214493148637	3.233504022912767E-4
26	6.940203756495696E-4	0.0	0.0	0.00453479222725571	3.3912359264694875E-4
27	7.097935660052416E-4	0.0	0.0	0.006537987402426059	3.470101878247848E-4
28	7.255667563609137E-4	0.0	0.0	0.013982933250303258	3.470101878247848E-4
29	7.255667563609137E-4	0.0	0.0	0.029503752560284542	3.470101878247848E-4
30	7.255667563609137E-4	0.0	0.0	0.0493543126228978	3.470101878247848E-4
31	7.571131370722577E-4	0.0	0.0	0.07702837510192438	3.470101878247848E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	23925	0.0	20.815884	1
GTATCAA	44975	0.0	16.922514	1
TATCAAC	65660	0.0	11.580109	1
AACGCAG	66610	0.0	11.514938	5
ATCAACG	67700	0.0	11.266691	2
TCAACGC	68565	0.0	11.170423	3
CAACGCA	69225	0.0	11.069267	4
ACGCAGA	70945	0.0	10.850447	6
TTATACT	2515	0.0	10.813122	4
CGCAGAG	72055	0.0	10.683298	7
AGAGTAC	73240	0.0	10.533179	10
GTATTAT	1795	0.0	10.512535	1
CAGAGTA	75110	0.0	10.381773	9
GCAGAGT	75950	0.0	10.210928	8
TACGGAT	2040	0.0	10.156862	27
CGTTATA	295	2.8559953E-8	10.033898	2
TATACAG	3195	0.0	10.017214	5
ATTATAC	1960	0.0	10.005102	3
TATACTG	2915	0.0	9.96398	5
GTATTAC	1310	0.0	9.885496	1
>>END_MODULE