FastQCFastQC Report
Thu 26 May 2016
SRR1041729_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041729_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16070957
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1536380.9559978288785166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1486750.9251160338491354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1017010.6328247907078589No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400850.24942509646438604No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT256260.1594553454408471No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT170320.10597999857755826No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167850.10444306459161082No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164130.10212833000548754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA547650.015.5154761
GTATCAA899750.014.0680191
TATACCG9200.011.4619565
ACGGATG32950.011.060698528
GTTACGG25800.010.9709325
TACGGAT33350.010.81709127
ATACCGT11250.010.68888956
TCTATAC25300.010.675893
AACGCAG1214000.010.5955935
TATCAAC1238800.010.3685431
TTATACT36950.010.263874
TCGTTAC21250.010.18588323
TAATACC20950.010.1551324
TATACTG40300.010.05334955
ATCAACG1277400.010.0436442
ATTATAC26450.010.0018913
GGTTGTG82600.09.96670734
TACCGTC9100.09.9615397
ACGCAGA1293800.09.9592296
CGTTACG18050.09.94182824
Produced by FastQC (version 0.11.5)