FastQCFastQC Report
Thu 26 May 2016
SRR1041728_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1041728_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25566624
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1925780.7532398489530726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1832420.7167234907510667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1346900.5268196536234115No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT544980.21316072078972959No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319890.12512015665423795No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269560.10543433501427486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGTA109850.017.0769231
TATGTAA113350.016.3700942
GGTATCA801850.015.7579351
GTATCAA1277700.013.2151131
CCGATAA15050.012.9069789
CGATAAC15000.012.82666810
ATGTAAG150150.012.5181483
TACGGAT84750.012.35516327
ACGGATG86300.012.19756728
TGTAAGT161850.012.0475764
TCGTTAC49800.011.62750923
GTTACGG62300.011.40288825
TTACGGA94800.011.29905126
GGGTAGA45550.011.2502751
GTAAGTA165300.011.2253485
CGTTACG42550.011.17391324
AACGCAG1650500.011.0641325
CATTACG39800.010.87688424
TACTTAC60100.010.83527631
TATACAC94350.010.80392237
Produced by FastQC (version 0.11.5)