##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1041728_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25566624
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51168445235476	34.0	31.0	34.0	31.0	34.0
2	32.710229946667965	34.0	31.0	34.0	31.0	34.0
3	32.7717528915824	34.0	31.0	34.0	31.0	34.0
4	36.16267466521978	37.0	35.0	37.0	35.0	37.0
5	36.13920731184532	37.0	35.0	37.0	35.0	37.0
6	36.21924032676352	37.0	37.0	37.0	35.0	37.0
7	36.21738975001158	37.0	37.0	37.0	35.0	37.0
8	36.17203620626642	37.0	37.0	37.0	35.0	37.0
9	37.94101602933575	39.0	38.0	39.0	35.0	39.0
10	37.88399946743066	39.0	38.0	39.0	35.0	39.0
11	37.95316921780521	39.0	38.0	39.0	35.0	39.0
12	37.88052489839879	39.0	38.0	39.0	35.0	39.0
13	37.88954106729148	39.0	38.0	39.0	35.0	39.0
14	38.76130219617576	40.0	39.0	40.0	36.0	40.0
15	38.74735283782481	40.0	39.0	40.0	36.0	40.0
16	38.725528407661486	40.0	39.0	40.0	36.0	40.0
17	38.73268895416149	40.0	39.0	40.0	36.0	40.0
18	38.70965439160055	40.0	39.0	40.0	36.0	40.0
19	38.71633442882408	40.0	39.0	40.0	36.0	40.0
20	38.68231965237178	40.0	39.0	40.0	36.0	40.0
21	38.651203146727546	40.0	39.0	40.0	36.0	40.0
22	38.560300257085174	40.0	39.0	40.0	35.0	40.0
23	38.509206534269055	40.0	38.0	40.0	35.0	40.0
24	38.47979181764475	40.0	38.0	40.0	35.0	40.0
25	38.41326367532921	40.0	38.0	40.0	35.0	40.0
26	38.2973563111031	40.0	38.0	40.0	35.0	40.0
27	38.2124404457937	40.0	38.0	40.0	34.0	40.0
28	38.12715781324902	40.0	38.0	40.0	34.0	40.0
29	38.04789701604717	40.0	38.0	40.0	34.0	40.0
30	37.959371992172294	40.0	38.0	40.0	34.0	40.0
31	38.0177623373348	40.0	38.0	40.0	34.0	40.0
32	37.98139269384961	40.0	38.0	40.0	34.0	40.0
33	37.936262957518366	40.0	38.0	40.0	34.0	40.0
34	37.94014258589636	40.0	38.0	40.0	34.0	40.0
35	37.92941297216246	40.0	38.0	40.0	34.0	40.0
36	37.90742270078365	40.0	38.0	40.0	34.0	40.0
37	37.85791041476575	40.0	38.0	40.0	34.0	40.0
38	37.79098464466799	40.0	38.0	40.0	34.0	40.0
39	37.712096638179524	40.0	38.0	40.0	34.0	40.0
40	37.656707471428376	40.0	38.0	40.0	33.0	40.0
41	37.57118080979327	40.0	37.0	40.0	33.0	40.0
42	37.482646633360744	40.0	37.0	40.0	33.0	40.0
43	37.227009166325594	40.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	8.0
12	30.0
13	29.0
14	59.0
15	54.0
16	117.0
17	251.0
18	478.0
19	819.0
20	1775.0
21	3909.0
22	7647.0
23	14339.0
24	25695.0
25	42736.0
26	67997.0
27	102000.0
28	149641.0
29	206728.0
30	276936.0
31	357779.0
32	457002.0
33	588094.0
34	791882.0
35	1108182.0
36	1701515.0
37	3063400.0
38	1.0848099E7
39	5749415.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.44760778740283	18.94443709110753	12.368500432438791	23.239454689050852
2	18.45917943644026	23.369972507907182	35.70048591476137	22.470362140891186
3	21.852916521164467	24.502714945860664	29.88358572488882	23.76078280808604
4	15.36570882412946	15.694715892094319	37.045219580027464	31.89435570374876
5	15.085061680415842	34.61821552974691	34.326925604256544	15.9697971855807
6	30.74162235890042	34.07131109684251	18.12751656221799	17.059549982039083
7	28.246244009377225	31.881886321792035	20.311375487041232	19.560494181789508
8	27.63698093264093	33.15406054393416	19.24102689506444	19.967931628360475
9	27.301774375842502	15.986702037781757	19.60962855322627	37.10189503314947
10	17.108535722197814	27.379195626297786	32.41987678936413	23.092391862140264
11	33.58642110902089	21.97869769587099	23.771363008272033	20.66351818683609
12	24.189290693992294	24.689646157427745	27.970102740197532	23.150960408382428
13	29.854195845333354	22.10729504216122	24.552256097637297	23.486253014868133
14	23.070726897692868	21.445353911412006	27.103226456492653	28.38069273440248
15	25.14354652378038	27.28426717583049	24.475335499908006	23.09685080048113
16	23.94993175477529	26.29757452528734	25.399689063366367	24.352804656571003
17	24.210443271665433	25.7168369198843	25.9597825665211	24.112937241929163
18	24.478769664700355	24.807671126230822	27.142426000398018	23.571133208670805
19	26.10845686939347	24.522158263836477	27.125051786266347	22.244333080503704
20	24.91628147697561	24.473817896332342	27.24827884980043	23.36162177689162
21	24.564627695858476	24.887724714846982	27.834496255743424	22.713151333551117
22	24.398309295744326	24.589496055482336	26.91514530819556	24.09704934057778
23	23.60384773523481	24.84058122026592	27.63101221342325	23.924558831076016
24	23.265308708729005	24.91251875883183	27.55192472811428	24.27024780432489
25	24.024787160009865	24.646425746316762	27.56699515743651	23.76179193623687
26	23.597362717893454	24.94593732829176	27.64089619341216	23.815803760402627
27	23.791608152879316	25.583475549998308	27.171780677808695	23.45313561931368
28	23.744394254008665	25.507478030732567	26.87917653891261	23.86895117634616
29	23.79265639452436	25.657642557734643	27.350126477394905	23.199574570346087
30	24.185230713292455	25.555349036306087	27.026642234813636	23.232778015587822
31	23.985274708150754	24.964394986213275	27.512412276255166	23.537918029380805
32	23.579292283564698	25.78688527667947	27.04721202142293	23.586610418332903
33	23.462792741036125	25.115858081223397	27.37795181718165	24.04339736055883
34	23.872522238368273	25.46035409289862	27.410392549286133	23.25673111944698
35	23.703227301344125	25.5875629101441	27.100457221102015	23.60875256740976
36	23.70557019964779	25.40541527891989	27.17435434572824	23.714660175704076
37	23.32299720135126	25.65207279615799	27.367082177138446	23.657847825352302
38	23.94169445289296	25.347355208102563	26.954035855496606	23.756914483507874
39	23.29141696611958	25.905782476403612	27.285033800317166	23.51776675715965
40	24.076882422958935	25.082901050995236	27.437650743406717	23.402565782639115
41	23.487469444538316	25.21225719907329	27.429386062078436	23.870887294309956
42	23.305771618497616	25.391248371314102	27.483961120560927	23.81901888962735
43	23.277347842249334	24.718378930280352	27.69222874322398	24.312044484246336
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52798.0
1	40015.5
2	27233.0
3	36700.5
4	46168.0
5	46168.0
6	54090.0
7	62012.0
8	51189.5
9	40367.0
10	52381.5
11	64396.0
12	64396.0
13	97442.0
14	130488.0
15	225421.0
16	320354.0
17	306695.5
18	293037.0
19	293037.0
20	276772.5
21	260508.0
22	215185.0
23	169862.0
24	195988.0
25	222114.0
26	222114.0
27	257322.5
28	292531.0
29	346365.5
30	400200.0
31	462661.0
32	525122.0
33	525122.0
34	602533.0
35	679944.0
36	797539.0
37	915134.0
38	1039383.5
39	1163633.0
40	1163633.0
41	1300137.0
42	1436641.0
43	1586849.0
44	1737057.0
45	1876830.0
46	2016603.0
47	2016603.0
48	2147723.5
49	2278844.0
50	2331111.0
51	2383378.0
52	2344074.0
53	2304770.0
54	2304770.0
55	2157320.0
56	2009870.0
57	1827603.5
58	1645337.0
59	1501999.5
60	1358662.0
61	1358662.0
62	1173002.5
63	987343.0
64	820652.5
65	653962.0
66	540700.0
67	427438.0
68	427438.0
69	345894.5
70	264351.0
71	216186.5
72	168022.0
73	132710.0
74	97398.0
75	97398.0
76	74761.5
77	52125.0
78	42077.0
79	32029.0
80	26824.5
81	21620.0
82	21620.0
83	17394.0
84	13168.0
85	10325.5
86	7483.0
87	5291.5
88	3100.0
89	3100.0
90	2009.0
91	918.0
92	612.5
93	307.0
94	266.0
95	225.0
96	225.0
97	127.5
98	30.0
99	36.0
100	42.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2.5566624E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.85623561632682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.917238666239996	13.101185850198751
2	18.41102867356461	8.784356759510127
3	8.572447966177641	6.1351901546950645
4	4.708656132591475	4.493232405414578
5	2.7749586207847274	3.3100033341498887
6	1.8418637141341139	2.636396084252775
7	1.337356954212834	2.2333011821984203
8	1.0076477991776083	1.9230946652363357
9	0.7579601241552499	1.6273867778665179
>10	4.829321484928957	22.15268678255738
>50	0.48086621283935604	8.008387728229737
>100	0.3304869232933677	14.705908345617319
>500	0.020499803271521167	3.3271379917048622
>1k	0.008978917310380521	3.7723155465020817
>5k	4.5867154533903534E-4	0.7657999430408282
>10k+	2.293357726686864E-4	3.023616448825213
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	192578	0.7532398489530726	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	183242	0.7167234907510667	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	134690	0.5268196536234115	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54498	0.21316072078972959	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31989	0.12512015665423795	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26956	0.10543433501427486	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9556747109043414E-5	0.0	0.0	1.1734048265426049E-5	2.3468096530852098E-5
2	4.6936193061704196E-5	0.0	0.0	3.911349421808683E-5	3.520214479627815E-5
3	6.649294017074761E-5	0.0	0.0	8.213833785798235E-5	3.520214479627815E-5
4	6.649294017074761E-5	0.0	0.0	1.9556747109043417E-4	5.084754248351288E-5
5	2.4250366415213835E-4	0.0	0.0	2.2294691704309493E-4	6.258159074893893E-5
6	3.481100985409728E-4	0.0	0.0	2.307696158867123E-4	1.2516318149787786E-4
7	3.676668456500162E-4	0.0	0.0	2.620604112611818E-4	1.368972297633039E-4
8	4.7718462946065934E-4	0.0	0.0	2.816171583702252E-4	1.564539768723473E-4
9	5.358548707877895E-4	0.0	0.0	5.319435213659809E-4	1.9556747109043417E-4
10	8.839649693287623E-4	0.0	0.0	7.353336913000324E-4	2.268582664649036E-4
11	0.001024773548513875	0.0	0.0	8.839649693287623E-4	2.307696158867123E-4
12	0.001075621090997388	0.0	0.0	9.85660054295788E-4	2.34680965308521E-4
13	0.0011421140311681354	0.0	0.0	0.001059975693310153	2.4250366415213835E-4
14	0.0013494155505239956	3.911349421808683E-6	0.0	0.0011069118863718573	2.542377124175644E-4
15	0.001408085791851126	3.911349421808683E-6	0.0	0.0011812275253862223	2.933512066356512E-4
16	0.0014980468285527256	3.911349421808683E-6	0.0	0.0012868339597750568	3.2073065258831203E-4
17	0.0015254262745053864	3.911349421808683E-6	0.0	0.0014745787320218735	3.2073065258831203E-4
18	0.0015488943710362385	3.911349421808683E-6	0.0	0.0017561958903920987	3.3637605027554674E-4
19	0.0015840965158325166	3.911349421808683E-6	0.0	0.0020534584464495585	3.3637605027554674E-4
20	0.001826600179984655	3.911349421808683E-6	0.0	0.002667540305673522	3.3637605027554674E-4
21	0.002409391243834149	3.911349421808683E-6	0.0	0.0042007892790225255	3.481100985409728E-4
22	0.002456327436895853	3.911349421808683E-6	0.0	0.006574978378060396	3.637554962282075E-4
23	0.002483706882848514	3.911349421808683E-6	0.0	0.008851383741553049	4.4589383408618987E-4
24	0.002542377124175644	3.911349421808683E-6	0.0	0.013208626997447922	4.576278823516159E-4
25	0.0026049587149245827	3.911349421808683E-6	0.0	0.017354657384565125	4.654505811952333E-4
26	0.0028318169813894866	3.911349421808683E-6	0.0	0.025294696710836753	4.850073283042767E-4
27	0.0028826645238729993	3.911349421808683E-6	0.0	0.04437425919041951	4.889186777260854E-4
28	0.003027384452479921	3.911349421808683E-6	0.0	0.10414750105449981	4.92830027147894E-4
29	0.003062586597276199	3.911349421808683E-6	0.0	0.20773567914168098	4.967413765697027E-4
30	0.003093877392650668	3.911349421808683E-6	0.0	0.33039559700960125	4.967413765697027E-4
31	0.0031994838270395028	3.911349421808683E-6	0.0	0.46434366930886145	5.162981236787462E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGTA	10985	0.0	17.076923	1
TATGTAA	11335	0.0	16.370094	2
GGTATCA	80185	0.0	15.757935	1
GTATCAA	127770	0.0	13.215113	1
CCGATAA	1505	0.0	12.906978	9
CGATAAC	1500	0.0	12.826668	10
ATGTAAG	15015	0.0	12.518148	3
TACGGAT	8475	0.0	12.355163	27
ACGGATG	8630	0.0	12.197567	28
TGTAAGT	16185	0.0	12.047576	4
TCGTTAC	4980	0.0	11.627509	23
GTTACGG	6230	0.0	11.402888	25
TTACGGA	9480	0.0	11.299051	26
GGGTAGA	4555	0.0	11.250275	1
GTAAGTA	16530	0.0	11.225348	5
CGTTACG	4255	0.0	11.173913	24
AACGCAG	165050	0.0	11.064132	5
CATTACG	3980	0.0	10.876884	24
TACTTAC	6010	0.0	10.835276	31
TATACAC	9435	0.0	10.803922	37
>>END_MODULE