FastQCFastQC Report
Wed 27 Apr 2016
SRR616892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616892_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57654199
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT2278000.39511432636502336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1214550.21066115236463523No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT694950.12053762120604608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT680700.030.15339720
GCGATTT708550.028.9872521
CGATTTT713050.028.81693522
ATAGAAT795200.027.6779042
TATAGAA821950.026.8564551
GAATTCG855550.025.945145
TAGAATT880850.025.1110523
AATTCGC883800.025.0340166
AGAATTC903050.024.4698684
ATTCGCG945900.023.644997
TTCGCGG1021850.022.1914148
TCGCGGC1022050.022.1037469
GATTTTT965050.021.53947423
TCGCGAT1009650.021.5035819
CTCGCGA1021950.021.26678318
CCGCTCG1068100.021.01593815
GCTCGCG1064350.020.9839817
CGCGGCC1083400.020.83753610
CGCTCGC1079700.020.72316216
CTCGTAT79300.020.20054242
Produced by FastQC (version 0.11.5)