FastQCFastQC Report
Wed 27 Apr 2016
SRR616891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616891_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46570480
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1771580.38040836169178416No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1173880.2520652567892794No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT763880.16402665379442086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT541050.029.19132220
GCGATTT564100.028.01846121
CGATTTT567350.027.91347922
ATAGAAT611300.027.7772352
CTCGTAT84800.027.11505742
TATAGAA638900.026.7209051
GAATTCG673150.025.4015665
TCTCGTA90150.025.03193541
TAGAATT687750.024.835693
TCGTATG95650.024.58027643
AATTCGC696150.024.4651056
AGAATTC701600.024.2893774
CGTATGC99650.023.70647644
ATTCGCG739350.023.2942757
ACACGTC111000.022.9244713
ATCTCGT105650.021.74279840
ACGTCTG117850.021.6301515
TTCGCGG808550.021.5789098
TCGCGGC812500.021.421649
CGTCTGA121550.020.98998616
Produced by FastQC (version 0.11.5)