FastQCFastQC Report
Wed 27 Apr 2016
SRR616890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616890_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51610324
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT2010720.3895964691095526No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1165950.2259141019924618No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT709300.13743374290771745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT610700.029.89065420
GCGATTT633050.028.84602221
CGATTTT639400.028.61235622
ATAGAAT701900.027.89952
TATAGAA726650.027.0278241
GAATTCG758850.025.9039755
TAGAATT774250.025.4229183
AATTCGC781650.025.0790066
AGAATTC798650.024.5940864
ATTCGCG839400.023.6082217
TTCGCGG906650.022.164838
TCGCGGC906350.022.1325579
TATCTCG59600.021.89458339
TCGCGAT905500.021.3309919
GATTTTT867050.021.30010823
CTCGCGA914350.021.13908418
CGCGGCC961600.020.92173410
CCGCTCG956300.020.85415615
GCTCGCG950700.020.81833617
CGCTCGC965650.020.57291816
Produced by FastQC (version 0.11.5)