FastQCFastQC Report
Wed 27 Apr 2016
SRR616889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616889_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62642682
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT2049750.3272130015122916No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1236380.1973702211536856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT658250.028.66604220
CGATTTT689850.027.41199122
ATAGAAT731650.027.3759962
GCGATTT689150.027.36113221
TATAGAA754750.026.7975881
GAATTCG823300.024.7889845
TAGAATT829950.024.5398833
AATTCGC847900.023.9762256
AGAATTC876300.023.2281154
ATTCGCG922050.022.3116137
CTCGTAT84900.021.22628642
AATCTCG85150.021.1913739
TTCGCGG991400.020.9732748
TCGCGGC995200.020.9407679
TCGCGAT1003100.020.10736819
CTCGCGA1014950.019.89920618
CGCGGCC1060350.019.71141210
GATTTTT973700.019.70295723
TCTCGTA90100.019.6272841
GCTCGCG1055750.019.53091817
Produced by FastQC (version 0.11.5)