FastQCFastQC Report
Wed 27 Apr 2016
SRR616888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616888_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50154758
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT2128870.42446022768168873No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1141490.22759356151215004No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT654280.1304522294774107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT632300.030.51158120
GCGATTT655300.029.42007321
CGATTTT659550.029.2579922
ATAGAAT712450.028.4615782
TATAGAA738650.027.6770571
GAATTCG787400.026.1792055
TAGAATT792700.025.9462033
AATTCGC809050.025.3696446
AGAATTC820600.025.0160564
CTCGTAT77300.024.94326842
ATTCGCG871850.023.8596577
TCTCGTA83250.022.94481841
TTCGCGG930750.022.6712538
TCGCGGC932100.022.655529
TCGTATG86750.022.64106243
CGTATGC87850.022.43439744
GATTTTT879850.022.13192223
TCGCGAT938800.021.88012319
CTCGCGA950500.021.62972818
CGCGGCC988450.021.41176210
Produced by FastQC (version 0.11.5)