FastQCFastQC Report
Wed 27 Apr 2016
SRR616887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616887_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49481268
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1582330.3197836401443876No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1017030.20553838676890818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT506150.028.5194320
GCGATTT528650.027.32688521
ATAGAAT569000.027.2486612
CGATTTT532800.027.12278422
TATAGAA598300.026.1837121
GAATTCG640600.024.6799835
TAGAATT650000.024.22313
AATTCGC662550.023.780326
AGAATTC673900.023.4197964
ATTCGCG724600.021.9705897
TCGCGGC782000.020.6082159
TTCGCGG782400.020.5631718
TCGCGAT779800.019.8296619
CTCGCGA789500.019.59172818
GCTCGCG818500.019.41167417
CGCGGCC832650.019.36813210
GATTTTT761350.019.21441323
CCGCTCG831250.019.21407515
CGCTCGC840700.018.99274416
GGCCGCT863750.018.54581313
Produced by FastQC (version 0.11.5)