FastQCFastQC Report
Wed 27 Apr 2016
SRR616886_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616886_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47106686
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT2219210.47110297676215224No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA974050.2067753184760227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT635400.031.53489320
GCGATTT659050.030.44081521
ATAGAAT696250.030.4161242
CGATTTT667200.030.12312122
TATAGAA716150.029.7687241
GAATTCG758200.028.4252195
TAGAATT770350.027.9135423
AATTCGC778200.027.5434026
AGAATTC796600.026.93064
ATTCGCG840050.025.794017
TCGCGGC901200.024.4234099
TTCGCGG899600.024.4216758
TCGCGAT898500.023.5175819
GATTTTT871700.023.30416523
CTCGCGA911900.023.17446718
GCTCGCG940400.023.04404817
CGCGGCC955050.023.0392410
CCGCTCG951200.022.929215
CGCTCGC963100.022.59201416
GGCCGCT985900.022.1723813
Produced by FastQC (version 0.11.5)