FastQCFastQC Report
Wed 27 Apr 2016
SRR616885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616885_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43451262
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1741440.4007800740056756No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA908850.2091653862665715No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT481780.11087825251197536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT523400.029.81895320
ATAGAAT586800.028.717462
GCGATTT544850.028.66979621
CGATTTT547450.028.57062122
TATAGAA606150.027.8963971
GAATTCG652200.026.0244645
TAGAATT662100.025.532473
AATTCGC677950.024.9526586
AGAATTC684250.024.6848564
ATTCGCG733300.023.3085277
TTCGCGG788000.021.9874738
TCGCGGC786350.021.9681329
TCGCGAT780400.021.20698219
GATTTTT747850.021.17063723
CTCGCGA792350.020.90701918
CGCGGCC832050.020.75342810
GCTCGCG821400.020.65516317
CCGCTCG829200.020.61312115
CGCTCGC839550.020.3024216
GGCCGCT861000.019.92235213
Produced by FastQC (version 0.11.5)