FastQCFastQC Report
Wed 27 Apr 2016
SRR616884_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616884_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62028065
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT8112981.3079531015516928No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT4766930.7685118018754897No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT1910900.3080702259533648No Hit
TGTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT887910.14314649344615216No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT870710.140373555099615No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA721240.11627639843351553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA3832150.034.2806241
GCGTTTT1793950.031.96668445
ATAGAAT4242900.030.957883
TATAGAA4244300.030.8462722
GAATTCG4311850.030.1152136
TAGAATT4387200.029.9692844
AATTCGC4335500.029.8440287
AGAATTC4371750.029.776715
CGCGATA2029100.029.67869421
ATTCGCG4418300.029.3214538
TAATACG1892600.029.3148926
CGCGATT1980000.029.21471421
GCGATAA1981300.029.18752722
TCGCGAT4244500.029.07494220
CGATAAT1977800.028.93904923
AATACGA1858750.028.93551327
TCGCGGC4443900.028.91306510
ATACGAC1937800.028.87708528
TTCGCGG4499800.028.8188849
TACGACT1973250.028.79640629
Produced by FastQC (version 0.11.5)