FastQCFastQC Report
Wed 27 Apr 2016
SRR616884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616884_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62028065
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1860160.2998900578310802No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1223260.1972107303363405No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690790.11136733025607039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT621750.027.28043620
ATAGAAT711050.026.434122
GCGATTT648200.026.11865621
CGATTTT652250.025.98062322
TATAGAA743600.025.3283081
GAATTCG799800.023.5594125
TAGAATT813050.023.2029973
CTCGTAT83050.022.918542
TATCTCG78800.022.89863239
AATTCGC832950.022.4890376
AGAATTC861400.021.9097484
ATTCGCG914800.020.7277287
TCTCGTA92800.020.43805541
TCGTATG96650.020.2289543
TTCGCGG989450.019.3662938
TCGCGGC990000.019.3150489
CGTATGC102900.018.9565344
TCGCGAT997650.018.49447319
ACCGATG109950.018.45831132
GATTTTT941950.018.32953623
Produced by FastQC (version 0.11.5)