FastQCFastQC Report
Wed 27 Apr 2016
SRR616883_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616883_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59339835
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT6810821.1477652406684313No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT4241660.7148081891363534No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1236150.20831706053783267No Hit
TATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTTT934920.1575535220143433No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920610.15514198851412378No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT677970.11425208715191068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA2951550.037.2745741
ATAGAAT3511100.031.363083
TATAGAA3525800.031.1404692
CGCGATA1597300.030.6079221
GAATTCG3563200.030.413676
TAGAATT3633600.030.2889144
TAATACG1497850.030.0905926
AATTCGC3591400.030.0602557
AGAATTC3624200.030.0346015
GCGATAA1574000.029.85268822
ATACGAC1527850.029.61741828
CGATAAT1571850.029.5948223
ATTCGCG3658650.029.5229388
TACGACT1556500.029.50455729
GCGTTTT1573600.029.49436845
AATACGA1480650.029.49419227
CGACTCA1554100.029.4067931
TCGCGAT3534750.029.17388320
ACGACTC1574300.029.15240530
TCGCGGC3690250.029.06854610
Produced by FastQC (version 0.11.5)