FastQCFastQC Report
Wed 27 Apr 2016
SRR616883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616883_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59339835
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT2847060.47978899840216943No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1158760.19527523121693882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT816750.031.22016320
GCGATTT850200.030.01566521
CGATTTT854700.029.87610622
ATAGAAT916300.029.5033632
TATAGAA949400.028.588511
GAATTCG993150.027.5042155
TAGAATT1002950.027.0552253
AATTCGC1030900.026.3745026
AGAATTC1044550.025.978574
ATTCGCG1095250.025.157687
TTCGCGG1171050.023.8002018
TCGCGGC1170400.023.7577539
GATTTTT1099450.023.42421223
TCGCGAT1175600.022.79149419
CGCGGCC1230050.022.62761310
CTCGCGA1186200.022.60284818
CCGCTCG1237250.022.31405415
GCTCGCG1231200.022.28838317
CGCTCGC1249000.022.05001816
GGCCGCT1289150.021.50649813
Produced by FastQC (version 0.11.5)