FastQCFastQC Report
Wed 27 Apr 2016
SRR616882_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616882_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41253396
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1169160.28340939495017575No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA701990.17016538468736003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT406600.026.93776720
ATAGAAT458550.026.123122
GCGATTT424700.025.75793621
CGATTTT430700.025.39388322
TATAGAA488700.024.6169851
GAATTCG513400.023.4760595
TAGAATT525400.022.9575353
AATTCGC537050.022.3958116
AGAATTC548550.021.9094284
ATTCGCG582700.020.834337
TCGCGGC637850.019.2271049
TTCGCGG639450.019.206988
TCGCGAT642500.018.55918919
GATTTTT604050.018.40851623
CTCGCGA650050.018.34001718
CGCGGCC677400.018.14440710
GCTCGCG676300.018.00408217
CCGCTCG683500.017.88701815
CGCTCGC690650.017.68540416
GCCGCTC718200.017.0791914
Produced by FastQC (version 0.11.5)