FastQCFastQC Report
Wed 27 Apr 2016
SRR616881_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616881_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45049951
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT6164441.3683566492669437No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT3797360.8429221154979725No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1084910.2408237913510716No Hit
TATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTTT779050.17293026578430684No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA769520.17081483618039897No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT732930.16269274077567808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA2620000.037.4837571
ATAGAAT3115050.031.533163
TATAGAA3119800.031.4189152
CGCGATA1429450.031.0559121
GAATTCG3149300.030.7452456
GCGTTTT1362500.030.717745
TAGAATT3199400.030.6961544
TAATACG1343150.030.68011526
GCGATAA1406850.030.48884622
AGAATTC3193650.030.4697385
AATTCGC3167600.030.4575777
CGATAAT1400850.030.3107623
ATACGAC1367500.030.23499728
AATACGA1323000.030.19739227
TACGACT1395050.030.05247929
ATTCGCG3221250.029.9488098
CGACTCA1383600.029.94351831
ATAATAC1378400.029.83512725
ACGACTC1406300.029.81543230
TCGCGAT3113450.029.65038920
Produced by FastQC (version 0.11.5)