FastQCFastQC Report
Wed 27 Apr 2016
SRR616881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616881_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45049951
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT2409540.5348596272613038No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA967810.21483042234607536No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT546510.12131200764236125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT679850.031.87730620
ATAGAAT736200.031.2922022
GCGATTT704750.030.7829521
CGATTTT708300.030.66361222
TATAGAA762450.030.2997261
GAATTCG801800.029.0342565
TAGAATT807100.028.6768763
AATTCGC826350.028.0050666
AGAATTC831900.027.8150564
ATTCGCG876600.026.6743347
TTCGCGG940850.025.1397198
TCGCGGC939150.025.1062539
GATTTTT899750.024.35959223
TCGCGAT936750.024.24127819
CGCGGCC982050.024.02325410
CTCGCGA947250.023.9841818
GCTCGCG978100.023.75675417
CCGCTCG985750.023.72310315
CGCTCGC993300.023.45210516
GCCGCTC1018200.023.01787214
Produced by FastQC (version 0.11.5)