FastQCFastQC Report
Wed 27 Apr 2016
SRR616880_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616880_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55537873
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT7782531.4013014146220544No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT5065330.9120496926484744No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT1410360.25394562733794285No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT883200.1590266159454828No Hit
TGTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT766560.1380247313396392No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA751730.1353544814364785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA3385550.036.619031
ATAGAAT3778950.032.8399283
TATAGAA3797550.032.5774652
GCGTTTT1651350.032.50387245
GAATTCG3804150.032.0874826
TAGAATT3884200.031.93374
AATTCGC3829050.031.7283977
AGAATTC3868700.031.6975145
CGCGATA1802700.031.55637221
ATTCGCG3879450.031.3148148
TAATACG1681800.031.0632926
TCGCGGC3901400.030.8866210
TCGCGAT3762300.030.85941320
GCGATAA1773450.030.77735922
TTCGCGG3954350.030.7120029
ATACGAC1709050.030.67059928
CTCGCGA3791650.030.64605919
AATACGA1650750.030.632127
CGCGGCC3936800.030.54964311
TGTATAG642100.030.546721
Produced by FastQC (version 0.11.5)