FastQCFastQC Report
Wed 27 Apr 2016
SRR616880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616880_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55537873
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1574490.2834984335824312No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1161080.20906094117072146No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT704240.1268035597978338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT523000.027.42983620
ATAGAAT596400.026.4809382
GCGATTT551700.025.99475321
CGATTTT553850.025.88571722
GAATTCG631350.025.1885815
TATAGAA635350.025.0064091
TAGAATT668900.023.7510263
AATTCGC668450.023.719856
AGAATTC691350.023.0155684
ATTCGCG723950.022.1127597
TCGCGGC787050.020.5000069
TTCGCGG793700.020.4161268
TCGCGAT794600.019.58317219
CTCGCGA801750.019.42817318
CGCGGCC835300.019.34009410
GCTCGCG836450.019.1063817
CCGCTCG846150.018.9777615
CGCTCGC857450.018.70404216
GATTTTT800950.018.16380923
GCCGCTC888150.018.14871214
Produced by FastQC (version 0.11.5)