FastQCFastQC Report
Wed 27 Apr 2016
SRR616879_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616879_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53466903
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT9654221.8056441383934283No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT6237171.1665478361445398No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT1095840.20495670003553412No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA813080.15207164701497672No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT770760.14415647003156326No Hit
TGTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT561680.10505190472692985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA3905050.039.214871
ATAGAAT4241400.036.1185233
TATAGAA4251600.035.9316522
GAATTCG4275000.035.235186
TAGAATT4348200.035.2057954
AGAATTC4326600.035.0245865
AATTCGC4290200.034.900547
ATTCGCG4346650.034.438368
CGCGATA2055850.034.3766921
GCGTTTT1948450.034.2785545
TAATACG1911650.034.02776326
TCGCGGC4365550.033.9944710
TTCGCGG4417300.033.873299
GCGATAA2006550.033.7902222
TCGCGAT4231250.033.7261920
CGCGGCC4393750.033.66388311
CGATAAT1999200.033.58758523
CTCGCGA4260400.033.5493119
AATACGA1875750.033.51165827
ATACGAC1944250.033.49279828
Produced by FastQC (version 0.11.5)