FastQCFastQC Report
Wed 27 Apr 2016
SRR616879_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616879_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53466903
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1364700.2552420139240158No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1346890.2519109812662985No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT865410.1618590102366692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT518900.026.8462122
TATAGAA550350.025.4910761
CGCGATT490750.025.32614920
GAATTCG578750.024.2394355
CGATTTT516550.024.05683522
GCGATTT516500.024.05480821
TAGAATT599400.023.415623
AGAATTC613900.022.8369034
AATTCGC614300.022.7963266
ATTCGCG672500.021.0476287
TTCGCGG742600.019.2304468
TCGCGGC745300.019.1034229
TCGCGAT765400.017.9491619
CGCGGCC796950.017.91333210
CTCGCGA769450.017.8517618
GCTCGCG802250.017.56783517
CCGCTCG814050.017.42097515
CGCTCGC822500.017.18455316
GATTTTT743900.016.98891623
GCCGCTC858400.016.57332814
Produced by FastQC (version 0.11.5)