FastQCFastQC Report
Wed 27 Apr 2016
SRR616878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616878_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52691383
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT2218330.421004322471475No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750180.3321567778928862No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1368910.25979769785127865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT671300.029.13930920
ATAGAAT749400.028.2810942
GCGATTT694950.028.1411921
CGATTTT700850.027.9299722
TATAGAA784150.027.166181
GAATTCG812700.026.2926335
TAGAATT824650.025.906173
AGAATTC840550.025.3813254
AATTCGC850750.025.0319656
ATTCGCG915550.023.5281417
TCGCGGC982400.022.1515549
TTCGCGG987700.022.0964728
TCGCGAT987550.021.1793819
CGCGGCC1029400.021.16639310
GATTTTT938700.021.07591823
CTCGCGA997150.021.000318
CCGCTCG1041600.020.75862915
GCTCGCG1034050.020.74265517
CGCTCGC1051250.020.50600616
GCCGCTC1078600.020.10076714
Produced by FastQC (version 0.11.5)