FastQCFastQC Report
Wed 27 Apr 2016
SRR616877_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616877_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46665741
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT8144761.745340334357918No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT5292001.1340224941461874No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1336660.28643282445681084No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT1143410.24502128874370602No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT699780.1499558316238887No Hit
TGTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT638740.13687557216759935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA3404350.037.881411
ATAGAAT3694650.034.915353
TATAGAA3705850.034.7272072
GCGTTTT1633750.034.2848845
GAATTCG3722500.034.173566
TAGAATT3784750.034.099954
AGAATTC3765600.033.9329875
AATTCGC3740450.033.842357
CGCGATA1767350.033.80436321
ATTCGCG3794000.033.400058
TAATACG1644500.033.27020326
GCGATAA1734850.033.09512322
TCGCGGC3810800.032.9936210
TCGCGAT3680450.032.92548820
TTCGCGG3856950.032.852599
CGATAAT1727600.032.85095623
AATACGA1616000.032.82647727
ATACGAC1679950.032.77283528
CGCGGCC3831950.032.69499611
CTCGCGA3716050.032.64457319
Produced by FastQC (version 0.11.5)