FastQCFastQC Report
Wed 27 Apr 2016
SRR616877_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616877_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46665741
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1692410.36266647946295333No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1305450.2797448346528988No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1171930.25113283854209023No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC588110.12602607124571322TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG89700.032.28231439
CTCGTAT101200.029.74797442
TCTCGTA102150.029.18475341
ACCGATG116200.027.35975632
CACCGAT116600.027.1501231
TCGTATG115250.026.68779243
GTCACCG122350.026.0396729
CCGATGT117150.025.8318933
ATAGAAT457250.025.5053162
CGCGATT412200.025.38718420
CGTATGC123400.025.07108144
TCACCGA126600.024.93447330
ACACGTC129250.024.82362613
ATCTCGT122350.024.47668640
GCGATTT434100.024.08569121
ACGTCTG134550.023.94614215
CGATTTT438350.023.91376322
TATAGAA493500.023.8198031
GAATTCG503300.023.237325
CGTCTGA141950.023.06236316
Produced by FastQC (version 0.11.5)