FastQCFastQC Report
Wed 27 Apr 2016
SRR616876_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616876_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52124257
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT8156991.5649124744358467No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT5429951.0417318754298983No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1379820.2647174423992269No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT1234690.23687435966713155No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT936580.17968217753204616No Hit
TGTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT658090.12625407782791034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA3621600.037.2253761
ATAGAAT4000500.033.761463
TATAGAA4009750.033.548952
GCGTTTT1716800.033.52102745
GAATTCG4023500.033.0132066
TAGAATT4114550.032.807594
CGCGATA1878350.032.79188521
AGAATTC4077250.032.7397735
AATTCGC4052600.032.6365557
TAATACG1733650.032.37492426
GCGATAA1826500.032.33166522
ATTCGCG4103550.032.212518
CGATAAT1819250.032.05660623
ATACGAC1762850.031.9536128
TCGCGAT3933400.031.89722320
AATACGA1706350.031.8816827
TCGCGGC4101950.031.85510
TACGACT1801450.031.70979929
TTCGCGG4168600.031.7072949
CGACTCA1787150.031.63744231
Produced by FastQC (version 0.11.5)