FastQCFastQC Report
Wed 27 Apr 2016
SRR616876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616876_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52124257
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1642790.31516804162791234No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1637120.3140802563382342No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1303420.2500601591309014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT531650.027.50804120
ATAGAAT590800.027.10332
GCGATTT556450.026.25779721
CGATTTT561600.026.0650822
TATAGAA622650.025.8481851
GAATTCG651250.024.7263975
TAGAATT683600.023.625393
AGAATTC682500.023.6173114
AATTCGC692350.023.2095286
ATTCGCG745550.021.7465117
TCGCGGC807300.020.2865839
TTCGCGG812850.020.2089658
TCGCGAT814950.019.45287719
CTCGCGA823550.019.26066618
CGCGGCC852100.019.2094910
GATTTTT779400.019.08730523
CCGCTCG861450.018.92265715
GCTCGCG858350.018.90968317
CGCTCGC870100.018.7035116
GCCGCTC905000.018.10654314
Produced by FastQC (version 0.11.5)