FastQCFastQC Report
Wed 27 Apr 2016
SRR616875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616875_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50263861
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1993170.3965413639831608No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1395910.27771642930494334No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT885050.17608078297049246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT638000.030.1520212
CGCGATT597650.029.91774620
GCGATTT619050.028.90532121
TATAGAA669550.028.7609181
CGATTTT626600.028.6144922
GAATTCG697100.027.783265
TAGAATT713300.027.1869643
AATTCGC730850.026.4046066
AGAATTC736350.026.238154
ATTCGCG786450.024.8010717
TCGCGGC855700.023.1042379
TTCGCGG863750.022.9462178
TCGCGAT867100.022.06615419
CTCGCGA870950.021.97377818
CGCGGCC905250.021.87938710
GCTCGCG905950.021.66131817
CCGCTCG915800.021.55365415
GATTTTT850200.021.32978623
CGCTCGC927900.021.2265216
GGCCGCT959100.020.65799713
Produced by FastQC (version 0.11.5)