FastQCFastQC Report
Wed 27 Apr 2016
SRR616874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616874_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55053810
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1698910.3085908132425349No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1475870.26807772250458234No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT958830.17416233317912058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT537700.027.49983220
GCGATTT564150.026.22646321
CGATTTT565400.026.16052422
ATAGAAT791350.020.862762
TATAGAA823650.020.0987221
GAATTCG851900.019.491475
TAGAATT868750.019.110833
GATTTTT784550.019.07100323
AATTCGC887100.018.6646046
AGAATTC892050.018.5839184
ATTCGCG945200.017.7315527
TCGCGAT958750.016.90365219
TCGCGGC1005250.016.8178189
TTCGCGG1016500.016.6936658
CTCGCGA981550.016.56831218
GCTCGCG1026600.016.255517
CCGCTCG1035850.016.24502415
CGCTCGC1047050.016.03687516
CGCGGCC1059550.015.98355610
GCCGCTC1084400.015.57788414
Produced by FastQC (version 0.11.5)