FastQCFastQC Report
Wed 27 Apr 2016
SRR616873_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616873_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52571402
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT10131871.9272588545384428No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT6778321.2893549995109508No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2164720.41176759942601493No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1086690.20670744143365247No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT873910.16623296445470487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA4151800.039.653461
ATAGAAT4563550.036.0738873
TATAGAA4570850.035.9235652
TAGAATT4672250.035.2196964
GAATTCG4606100.035.1547286
CGCGATA2121150.035.010521
AGAATTC4652850.034.996585
AATTCGC4626850.034.8343247
TAATACG1969400.034.58519426
GCGATAA2065600.034.44272622
GCGTTTT2037200.034.39925845
ATTCGCG4694400.034.3668448
AATACGA1932750.034.2746727
CGATAAT2058150.034.25705323
ATACGAC2012800.034.1131528
TCGCGGC4701800.033.96600310
TCGCGAT4506350.033.93391420
TACGACT2057100.033.9166229
ATAATAC2007850.033.88096225
TTCGCGG4766450.033.8432669
Produced by FastQC (version 0.11.5)