FastQCFastQC Report
Wed 27 Apr 2016
SRR616873_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616873_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52571402
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2591080.4928687273738676No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1894050.36028143209876734No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1788700.3402420197962383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT619200.028.6034492
CGCGATT584900.027.76953720
TATAGAA651350.027.2633131
GCGATTT608350.026.72869121
CGATTTT613650.026.53450622
GAATTCG683750.026.1737025
TAGAATT696750.025.6466033
AGAATTC719150.024.8227354
AATTCGC722200.024.677196
ATTCGCG788500.022.8333727
TTCGCGG863950.021.1127478
TCGCGGC866600.020.9858749
CGCGGCC916050.019.8972710
GATTTTT829900.019.8968523
TCGCGAT886800.019.71625319
CTCGCGA893350.019.58177218
GCTCGCG925950.019.3637517
CCGCTCG939350.019.20732115
CGCTCGC945350.019.02591516
GGCCGCT984050.018.44687313
Produced by FastQC (version 0.11.5)