FastQCFastQC Report
Wed 27 Apr 2016
SRR616872_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616872_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72370724
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1593870.2202368460484104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1455530.2011213816238732No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1259900.17408973274883915No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1060210.1464970835444454TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG160850.033.13820339
CGCCAAT174550.032.1219633
CTCGTAT169750.031.45406342
GTCACGC185750.030.07611529
TCTCGTA175950.029.94913941
TCGTATG186750.029.13295643
ACACGTC201100.028.93278713
CGTATGC189450.028.646544
AGTCACG200950.027.95788828
ACGCCAA213450.026.73172232
ATCTCGT203500.026.18203540
ACGTCTG222800.026.14512415
ATATCTC208800.025.7324938
CACGTCT229550.025.45472714
CACACGT232150.025.06303812
CGTCTGA233000.024.99091716
CCAATAT224650.024.5577135
TCACGCC237300.023.98814230
CGCGATT599200.023.69361920
GTATGCC237300.022.983945
Produced by FastQC (version 0.11.5)