FastQCFastQC Report
Wed 27 Apr 2016
SRR616871_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616871_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61552679
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT9709381.5774098150951317No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT6929121.125721920243309No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1844730.29969938432736615No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1190180.19335957741173215No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT866850.1408305883810516No Hit
GTATAGAATTCGCGGCCGCTCGCGATGTATAGAATTCGCGGCCGCTCGCGA753080.12234723365980545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA4748800.036.573481
CGCGATA2098200.035.0660421
TAATACG1938200.034.67416826
GCGATAA2050400.034.45344522
GCGTTTT1990600.034.3384445
CGATAAT2040700.034.18649323
AATACGA1910600.034.0981327
ATACGAC1986050.034.0363728
TACGACT2012150.033.96385629
ATAGAAT5145400.033.7533533
CGACTCA1998350.033.75256731
TATAGAA5136350.033.7222562
ACGACTC2023100.033.7099730
ATAATAC1995750.033.5852125
TCGCGAT4780450.033.18408620
TATAGGG2001900.033.09200339
TAGGGCG2054500.033.05844541
GACTCAC2054150.032.9802732
GAATTCG5180800.032.9378626
GATAATA2064100.032.89934524
Produced by FastQC (version 0.11.5)